{"product_id":"hisprep-ff-columns-for-scale-up-protein-purification-28936551","title":"HisPrep™ FF columns for scale-up protein purification","description":"\u003cp\u003eHisPrep FF 16\/10 is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow. The column is an excellent choice for preparative, scale-up purification of histidine-tagged recombinant proteins.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eOptimized for convenient scale-up purification of histidine-tagged proteins.\u003c\/li\u003e\n\u003cli\u003eCompatible with a wide range of reducing agents, detergents, denaturants, and other additives.\u003c\/li\u003e\n\u003cli\u003eNegligible leakage of Ni2+.\u003c\/li\u003e\n\u003cli\u003eHigh binding capacity, approx. 40 mg\/mL resin.\u003c\/li\u003e\n\u003cli\u003eConvenient and time-saving 20 mL prepacked HiPrep format.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eHisPrep FF 16\/10 columns are prepacked with Ni Sepharose 6 Fast Flow for scale-up purification of histidine-tagged proteins. Ni Sepharose 6 Fast Flow consists of 90 ?m beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni\u003csup\u003e2+\u003c\/sup\u003e ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni\u003csup\u003e2+\u003c\/sup\u003e ions ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-13253-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-14265-pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"20 mL","offer_id":45982409261135,"sku":"28936551","price":3089.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-7637-original-png_269c1430-a33a-4935-a9a7-4a9976376a14.png?v=1785960784","url":"https:\/\/www.danyel.co.il\/products\/hisprep-ff-columns-for-scale-up-protein-purification-28936551","provider":"danyel biotech","version":"1.0","type":"link"}