{"product_id":"hiscreen-imac-ff-columns-28950517","title":"HiScreen™ IMAC FF columns","description":"\u003cp\u003eHiScreen IMAC FF are prepacked with IMAC Sepharose 6 Fast Flow, a resin which is charged with the metal of choice for purification of histidine-tagged proteins. The HiScreen format is optimized for method optimization and parameter screening.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eFor optimizing purification of histidine-tagged proteins when Ni2+ is not the best choice of metal ion.\u003c\/li\u003e\n\u003cli\u003eConveniently charge with your metal ion of choice.\u003c\/li\u003e\n\u003cli\u003eOptimal bead size for scale-up.\u003c\/li\u003e\n\u003cli\u003eHigh binding capacity.\u003c\/li\u003e\n\u003cli\u003ePrepacked HiScreen columns for method optimization and process development.\u003c\/li\u003e\n\u003cli\u003eReproducible results, scalable to BioProcess columns packed with the same resin using the same linear fluid velocity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eIMAC Sepharose 6 Fast Flow consists of 90 μm beads of highly cross-linked agarose to which a chelating group has been covalently coupled. This chelating group can be charged with suitable metal ions, specific for your target protein of interest.\u003c\/p\u003e\u003cp\u003eThe bead size of IMAC Sepharose 6 Fast Flow enables high flow properties, making the resin an excellent choice for scaling up purification at laboratory scale and purification of histidine-tagged proteins at process scale.\u003c\/p\u003e\u003cp\u003eLow metal-ion leakage means that the activity of the purified protein is retained and the risk of precipitation reduced, which results in increased purity, activity, and yield of the target protein. Leakage of metal ions into the eluted target protein pool from IMAC Sepharose 6 Fast Flow is generally low under normal conditions.\u003c\/p\u003e\u003cp\u003eIMAC Sepharose 6 Fast Flow is highly stable and compatible with a wide range of common additives. This helps maintain biological activity and increase product yield, while at the same time greatly expanding the range of suitable operating conditions.\u003c\/p\u003e\u003cp\u003eThe resin is available prepacked in HiTrap, HiScreen, and HiPrep column formats for convenient purification of histidine-tagged proteins at laboratory scale. Initial purification using a 1 mL HiTrap IMAC FF column can be scaled up further with the 5 mL column. Purification can be scaled up even more using 20 mL HiPrep IMAC FF 16\/10 columns. Prepacked HiScreen IMAC FF with a 10 cm bed height is the format of choice for method optimization and process development.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-14071-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/28950368.pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"4.7 mL","offer_id":45982045700175,"sku":"28950517","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-7717-original-png_cc43d493-f03f-4f35-bbbb-842977d33073.png?v=1785957777","url":"https:\/\/www.danyel.co.il\/products\/hiscreen-imac-ff-columns-28950517","provider":"danyel biotech","version":"1.0","type":"link"}