{"product_id":"hiscreen-column-with-mabselect-vh3-protein-a-resin-17549315","title":"HiScreen™ column with MabSelect™ VH3 protein A resin","description":"\u003cp\u003ePrepacked column with MabSelect™ VH3 resin. This protein A ligand interacts only with the variable heavy chain of the VH3 sequence family of the human antibody for efficient separation of bispecific antibodies (bsAb) and fragments, such as fragment antigen-binding region (Fab), single-chain variable fragment (scFv), and variable heavy domain of heavy chain (VHH).\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eHigh binding capacity for bispecific antibodies and antibody fragments containing the VH3 sequence family.\u003c\/li\u003e\n\u003cli\u003eProvides good resolution for product-related impurities in the capture of bispecific antibodies.\u003c\/li\u003e\n\u003cli\u003eExcellent alkaline stability; stable when cleaned with 0.5 M NaOH, minimizing risk for bioburden incidents and providing a long resin lifetime.\u003c\/li\u003e\n\u003cli\u003eA single interaction with the Fab VH3 region is beneficial over a dual-interaction affinity ligand, as separation of unwanted mispaired antibodies and fragments from target bsAb may be more efficient.\u003c\/li\u003e\n\u003cli\u003eEasily measure protein A leakage using the VH3 ligand ELISA kit specifically designed for use with MabSelect™ VH3 resin.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eMabSelect™ VH3 chromatography resin in different formats\u003c\/p\u003e\n\n\u003cp\u003eTo support process development, manufacturing, and research, MabSelect™ VH3 resin is available in \u003ca href=\"\/shop\/chromatography\/resins\/affinity-antibody\/mabselect-vh3-protein-a-affinity-resin-p-41718\"\u003e25 mL, 200 mL, 1 L, 5 L, and 10 L containers\u003c\/a\u003e. The resin can also be ordered in prepacked formats such as \u003ca href=\"\/shop\/chromatography\/prepacked-columns\/affinity-antibody\/hitrap-column-with-mabselect-vh3-protein-a-resin-p-41720\"\u003eHiTrap™ columns\u003c\/a\u003e, HiScreen™ columns, \u003ca href=\"\/en\/us\/shop\/chromatography\/prepacked-columns\/affinity-antibody\/predictor-mabselect-vh3-filter-plates-p-41724\"\u003ePreDictor™ plates\u003c\/a\u003e, and ReadyToProcess™ columns. \u003ca href=\"https:\/\/info.cytivalifesciences.com\/antibody-capture.html?extcmp=cy23001-global-bp-pd-mabselect-vh3-lp-ms-ms-product-pages\" target=\"_blank\"\u003eContact us\u003c\/a\u003e to learn more.\u003c\/p\u003e\n\n\u003cp\u003eEngineered for specific VH3 interaction\u003c\/p\u003e\n\n\u003cp\u003eThe ligand of MabSelect™ VH3 resin has been engineered to have affinity only for the variable region of the VH3 heavy chain. Traditional protein A resins have affinity for both the Fc region and the Fab VH3 region of human antibodies. In the ligand used in the MabSelect™ VH3 resin, the Fc interaction has been knocked out, and VH3 interaction has been enhanced. In bioprocessing, an affinity ligand with a single interaction with the Fab VH3 region is beneficial over a dual-interaction affinity ligand, as separation of unwanted mispaired antibodies and fragments from the target bispecific antibody (bsAb) may be more efficient. The VH3 sequence family is the most common VH class for antibodies in commercialized biologics.\u003c\/p\u003e\n\n\u003cp\u003eEfficient separation of bsAb and antibody fragments\u003c\/p\u003e\n\n\u003cp\u003eHigh dynamic binding capacity (DBC) allows high mass throughput of processed antibody per resin volume unit. It also provides productivity and process economy benefits. For a typical mAb (with VH3 sequence), MabSelect™ VH3 resin shows similar binding capacity as MabSelect PrismA™ resin. MabSelect™ VH3 resin showed significantly higher (70%) binding capacity for Fab compared to MabSelect PrismA™ resin. For VHH, MabSelect™ VH3 resin showed a higher (11%–14%) DBC compared to MabSelect PrismA™ resin.\u003c\/p\u003e\n\n\u003cp\u003eAsymmetric bispecific antibodies can form product-related impurities such as homodimers, half antibodies, and an excess of light chains during cell culture. They are also prone to aggregation. The similarities between these impurities present extra challenges for downstream processes, especially post-capture. MabSelect™ VH3 resin and its novel selectivity enables separation in the capture step of asymmetric bispecific antibodies, where only one of the heavy chains includes the VH3 sequence family, from product-related entities.\u003c\/p\u003e\n\n\u003cp\u003eRobust resin designed for harsh cleaning over many cycles\u003c\/p\u003e\n\n\u003cp\u003eThe alkaline stability of the MabSelect™ VH3 resin was evaluated in an accelerated alkaline stability study and in a repeated CIP cycling study with 0.5 M NaOH. The resin showed excellent alkaline stability when cleaned with 0.5 M NaOH.\u003c\/p\u003e\n\n\u003cp\u003eResin toolbox for the diversified antibody pipeline\u003c\/p\u003e\n\n\u003cp\u003eManufacturing platform approaches used for many traditional monoclonal antibodies (mAbs) are being adapted to fit respective antibody variant molecules. To provide the required purity and yield, a chromatography resin must be selected based on the domains of the target molecule, the impurity profile, and the antibody regions that the resin interacts with. Explore Cytiva's range of high-flow, modern affinity resins, including MabSelect™ VH3 resin, \u003ca href=\"\/shop\/chromatography\/resins\/affinity-antibody\/mabselect-prisma-protein-a-chromatography-resin-p-09659\"\u003eMabSelect PrismA™ resin\u003c\/a\u003e, \u003ca href=\"\/shop\/chromatography\/resins\/affinity-antibody\/mabselect-vl-protein-l-resin-p-31823\"\u003eMabSelect™ VL resin\u003c\/a\u003e, and \u003ca href=\"\/solutions\/bioprocessing\/products-and-solutions\/downstream-bioprocessing\/capto-high-productivity-resins\"\u003eCapto™ polishing resins\u003c\/a\u003e.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/wZr3I-C_EUaFvMYpt54vJQ-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/29736918.pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"1 × 4.7 mL","offer_id":45982442029135,"sku":"17549315","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/gvuw1eQUgkCC41TIKVHjLA-original-png_3aa54922-b506-4289-b212-30c718f3ca6b.png?v=1785961150","url":"https:\/\/www.danyel.co.il\/products\/hiscreen-column-with-mabselect-vh3-protein-a-resin-17549315","provider":"danyel biotech","version":"1.0","type":"link"}