{"product_id":"hiscreen-capto-octyl-hic-column-17546510","title":"HiScreen™ Capto™ Octyl HIC column","description":"\u003cp\u003eThis column packed with Capto™ Octyl resin is designed for HIC process development.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003ePrepacked with Capto™ Octyl high-flow and high-capacity HIC resin for capture and intermediate purification processes\u003c\/li\u003e\n\u003cli\u003eDesigned for efficient process development: convenient method optimization and parameter screening are enabled by the 10 cm bed height\u003c\/li\u003e\n\u003cli\u003eSmall bed volume for fast results and minimal sample\/buffer consumption\u003c\/li\u003e\n\u003cli\u003eDelivers reproducible results, and scalable\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp class=\"-font--plus-1 -padding--title-md\"\u003eAbout HiScreen™ column format for process development\u003c\/p\u003e\n\n\u003cp\u003eHiScreen™ columns are part of the process development platform available from Cytiva. The columns are designed for method optimization and parameter screening. HiScreen™ columns have small bed volumes (4.7 mL), reducing the cost of sample and buffer consumption.\u003c\/p\u003e\n\n\u003cp\u003e\u003cem\u003eMethod optimization and parameter screening\u003c\/em\u003e\u003c\/p\u003e\n\n\u003cp\u003eWhen developing a new process, parameters such as selectivity, capacity, and binding and elution conditions must be screened at smaller scale to save time and money. We suggest starting with high‑throughput parallel screening of process conditions which is easily performed using \u003ca href=\"\/shop\/chromatography\/prepacked-columns\/hydrophobic-interaction\/predictor-capto-octyl-p-06070\"\u003e96‑well PreDictor™ plates\u003c\/a\u003e prefilled with BioProcess™ resin. During the process development, HiScreen™ prepacked column format is available for further method optimization, or for the fine-tuning and verification of parameters.\u003c\/p\u003e\n\n\u003cp\u003e\u003cem\u003eScale-up\u003c\/em\u003e\u003c\/p\u003e\n\n\u003cp\u003eScale‑up of a chromatography step can be as straightforward as increasing column diameter to accommodate a larger feed volume while keeping the bed height and the linear flow velocity constant. The 10 cm bed height of HiScreen™ columns gives sufficient residence time to serve as the basis for linear process scale‑up. However, when needed, two columns can easily be connected in series to increase the bed height.\u003c\/p\u003e\n\n\u003cp class=\"-font--plus-1 -padding--title-md\"\u003eCapto™ Octyl – a high-flow and high-capacity HIC resin\u003c\/p\u003e\n\n\u003cp\u003eCapto™ Octyl hydrophobic interaction chromatography resin has an octyl ligand for the capture and intermediate purification of monoclonal antibodies (mAbs), mAb variants, and other biomolecules. It combines high capacity, narrow specification range, high flow rate, and low backpressure to meet the demands of large-scale biopharmaceutical manufacturing.\u003c\/p\u003e\n\n\u003cp\u003eNot sure which HIC resin you should use? We recommend starting with one of the kits below:\u003c\/p\u003e\n\n\u003cul\u003e\n\t\u003cli class=\"-font--body\"\u003e\n\u003ca href=\"\/shop\/chromatography\/prepacked-columns\/hydrophobic-interaction\/predictor-capto-hic-screening-kit-p-10629\"\u003ePreDictor™ Capto™ HIC screening kit\u003c\/a\u003e, a set of 96-well plates prefilled with six Capto™ HIC resins for use in high-throughput process development (HTPD)\u003c\/li\u003e\n\t\u003cli class=\"-font--body\"\u003e\n\u003ca href=\"\/shop\/chromatography\/prepacked-columns\/hydrophobic-interaction\/hitrap-capto-hic-selection-kit-p-11316\"\u003eHiTrap™ Capto™ HIC selection kit\u003c\/a\u003e, a set of five Capto™ HIC resins in HiTrap™ columns for resin selection and condition screening\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cp class=\"-font--plus-1 -padding--title-md\"\u003eCapto™ Octyl is available in other formats (Figure 1):\u003c\/p\u003e\n\n\u003cp\u003eThese formats support various scales of work from process development screening to GMP manufacturing.\u003c\/p\u003e\n\n\u003cul\u003e\n\t\u003cli class=\"-font--body\"\u003e\n\u003ca href=\"\/shop\/chromatography\/resins\/hydrophobic-interaction\/capto-octyl-hydrophobic-interaction-chromatography-resin-p-05567\"\u003eBulk resin bottles and containers\u003c\/a\u003e of Capto™ Octyl resin for column packing\u003c\/li\u003e\n\t\u003cli class=\"-font--body\"\u003e\n\u003ca href=\"\/shop\/chromatography\/prepacked-columns\/hydrophobic-interaction\/predictor-robocolumn-capto-octyl-p-09932\"\u003ePreDictor™ Capto™ Octyl RoboColumn™ units\u003c\/a\u003e, miniaturized columns for HTPD\u003c\/li\u003e\n\t\u003cli class=\"-font--body\"\u003e\n\u003ca href=\"\/shop\/chromatography\/prepacked-columns\/hydrophobic-interaction\/predictor-capto-octyl-p-06070\"\u003ePreDictor™ Capto™ Octyl multiwell plates\u003c\/a\u003e, 96-well plates for high-throughput screening or process development\u003c\/li\u003e\n\t\u003cli class=\"-font--body\"\u003e\n\u003ca href=\"\/shop\/chromatography\/prepacked-columns\/hydrophobic-interaction\/hitrap-capto-octyl-p-39717\"\u003eHiTrap™ Capto™ Octyl 1 mL and 5 mL columns\u003c\/a\u003e for easy use with a syringe, peristaltic pump, or chromatography system\u003c\/li\u003e\n\t\u003cli class=\"-font--body\"\u003e\n\u003ca href=\"\/shop\/chromatography\/prepacked-columns\/hydrophobic-interaction\/readytoprocess-capto-octyl-columns-p-23916\"\u003eReadyToProcess™ Capto™ Octyl columns\u003c\/a\u003e, in various sizes for scale-up and GMP manufacturing\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cp\u003e\u003cimg alt=\"\" class=\"img-responsive center-block\" src=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/HOUmC5F8SUKvHijilL28QQ-large?v=9560cba5\" style=\"max-width: 850px;\"\u003e\u003c\/p\u003e\n\n\u003cp class=\"-font--minus-1\"\u003e\u003cstrong\u003eFig 1.\u003c\/strong\u003e Prepacked chromatography formats available from Cytiva to support various scales, from screening to GMP manufacturing.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-14385-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/28995533.pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"1 × 4.7 mL","offer_id":45982415880271,"sku":"17546510","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/hBzSpoI9xkieo2SiTdFhMg-original-png_00d915a6-fd28-4927-800e-f320182dc705.png?v=1785960866","url":"https:\/\/www.danyel.co.il\/products\/hiscreen-capto-octyl-hic-column-17546510","provider":"danyel biotech","version":"1.0","type":"link"}