{"product_id":"hiprep-sp-ff-cation-exchange-chromatography-column-28936544","title":"HiPrep™ SP FF cation exchange chromatography column","description":"\u003cp\u003eHiPrep SP FF is a 20 mL strong cation exchange chromatography column for small scale protein purification.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003ePacked with SP Sepharose Fast Flow strong cation exchange resin.\u003c\/li\u003e\n\u003cli\u003eConvenient prepacked ion exchange chromatography column.\u003c\/li\u003e\n\u003cli\u003eFast, reliable, and reproducible preparative ion exchange separation.\u003c\/li\u003e\n\u003cli\u003eFeature good flow rates and high capacity.\u003c\/li\u003e\n\u003cli\u003e20 mL HiPrep column format compatible with single-pump configurations and chromatography systems such as ÄKTA systems.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eStrong cation exchange chromatography columns\u003c\/p\u003e\u003cp\u003eHiPrep SP FF columns are packed with SP Sepharose Fast Flow, which is based on 6% highly cross-linked agarose with an average bead size of 90 µm. This Sepharose resin has good flow properties and high loading capacities, making it excellent for ion exchange separation.\u003c\/p\u003e\u003cp\u003eThe functional groups of this SP resin are coupled to the matrix via chemically stable ether linkages and remain charged over the entire working pH range. They also maintain high capacity for cation exchange separations.\u003c\/p\u003e\u003cp\u003eSuccessful use in published research\u003c\/p\u003e\u003cp\u003eSP Sepharose Fast Flow resin, which is packed into HiPrep columns, is used in a variety of research applications, including:\n\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003ePurification of pro-form lipase from \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC3907806\/\"\u003e \u003ci\u003eRhizopus oryzae \u003c\/i\u003ein Pichia pastoris\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003ePurification of solid-phase PEGylated \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC3344868\/\"\u003erecombinant human keratinocyte growth factor 1\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eDevelopment of a lentiviral vector platform for production of bioengineered \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC3034847\/\"\u003erecombinant coagulation factor VIII\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eOther SP Sepharose Fast Flow formats\u003c\/p\u003e\u003cp\u003eThis cation resin is available in the following formats:\n\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eBulk resin SP Sepharose Fast Flow\u003c\/li\u003e\n\u003cli\u003eHiTrap SP FF columns convenient for easy use with a syringe, peristaltic pump, or chromatography system\u003c\/li\u003e\n\u003cli\u003eHiScreen SP FF columns for method and process development\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-14324-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-14402-pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"16 mm \/ 20 mL","offer_id":45982060085327,"sku":"28936544","price":4462.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-7641-original-png_e462763b-916d-4f00-b2c7-369e71bf2fcc.png?v=1785958102","url":"https:\/\/www.danyel.co.il\/products\/hiprep-sp-ff-cation-exchange-chromatography-column-28936544","provider":"danyel biotech","version":"1.0","type":"link"}