{"product_id":"hiprep-sephacryl-s-200-hr-preparative-sec-columns-17116601","title":"HiPrep Sephacryl™ S-200 HR preparative SEC columns","description":"\u003cp\u003eHiPrep Sephacryl S-200 HR (high-resolution) size exclusion chromatography columns are designed for routine protein purification at laboratory scale.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eBroad fractionation range: Mr ~ 5 000 to ~ 250 000 (globular proteins), Mr ~ 1 000 to ~ 80 000 (dextrans) suitable for varied applications, including purification of recombinant tagged proteins.\u003c\/li\u003e\n\u003cli\u003eHigh capacity columns: Preparative separation of protein in sample volumes up to 5 mL or 13 mL for 16\/60 and 26\/60 columns, respectively.\u003c\/li\u003e\n\u003cli\u003eConvenient: Pre-packed Sephacryl columns are easily connected to ÄKTA systems and other chromatography systems for automated chromatography at low to medium pressure.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eFast and reproducible, for routine protein purification\u003c\/p\u003e\n\n\u003cp\u003eSephacryl resins are dextran-acrylamide copolymers with extensive cross-linking, which gives good rigidity and chemical stability. The high resolution and flow characteristics, long-term physical and chemical stability, and ease of handling make Sephacryl resins an excellent option for routine purification by size exclusion chromatography (SEC). SEC is also known as gel filtration chromatography.\u003c\/p\u003e\n\n\u003cp\u003eBroad fractionation range (5 – 250 kDa) for diverse applications\u003c\/p\u003e\n\n\u003cp\u003eSephacryl S-200 HR resin has been used for decades to separate biomolecules across a wide molecular weight range. Examples include:\u003c\/p\u003e\n\n\u003cul\u003e\n\t\u003cli\u003eisolation and separation of correctly dimerized \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=sephacryl+s-200+hr+recombinant+tagged+proteins\" target=\"_blank\"\u003erecombinant tagged proteins\u003c\/a\u003e from monomer subunits following affinity purification\u003c\/li\u003e\n\t\u003cli\u003estudy of degradation of human immunoglobulins and hemoglobin by a molecular weight 60 000 cysteine proteinase of \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC2732966\/\" target=\"_blank\"\u003eTrichomonas vaginalis\u003c\/a\u003e\n\u003c\/li\u003e\n\t\u003cli\u003erelease of \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC4657934\/\" target=\"_blank\"\u003emonocyte caspase-1\u003c\/a\u003e in a stable, active high molecular weight complex\u003c\/li\u003e\n\t\u003cli\u003edifferential phosphorylation of plant translation initiation factors by \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC2742825\/\" target=\"_blank\"\u003eArabidopsis thaliana CK2 holoenzymes\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cp\u003eOther Sephacryl S-200 HR formats\u003c\/p\u003e\n\n\u003cp\u003eSephacryl S-200 HR is also available as bulk resin for packing columns of your own.\u003c\/p\u003e\n\n\u003cp\u003eOther Cytiva size exclusion chromatography columns\u003c\/p\u003e\n\n\u003cp\u003eLife Sciences solutions from Cytiva include a comprehensive range of size exclusion chromatography columns and resins. For higher resolution, HiLoad Superdex 200 pg and HiLoad Superdex 75 pg columns are available.\u003c\/p\u003e\n\n\u003cp\u003eNot sure which product to use? Let Cytiva help you \u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/dmm3bwsv3\/AssetStream.aspx?mediaformatid=10061\u0026amp;destinationid=10016\u0026amp;assetid=13947\"\u003eselect the right size exclusion chromatography product\u003c\/a\u003e for your needs.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-11195-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-17491-pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"16 mm","offer_id":45982029971535,"sku":"17116601","price":4650.0,"currency_code":"ILS","in_stock":true},{"title":"26 mm","offer_id":45982030004303,"sku":"17119501","price":6244.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-7707-original-png_11e3f2d5-39e8-40f7-b634-8b2199f09afd.png?v=1785957248","url":"https:\/\/www.danyel.co.il\/products\/hiprep-sephacryl-s-200-hr-preparative-sec-columns-17116601","provider":"danyel biotech","version":"1.0","type":"link"}