{"product_id":"hiprep-desalting-columns-with-sephadex-g-25-resin-17508702","title":"HiPrep™ desalting columns with Sephadex™ G-25 resin","description":"\u003cp\u003eHiPrep™ 26\/10 desalting are convenient, easy-to-use, 53 mL columns prepacked with Sephadex™ G-25 Fine resin for desalting and buffer exchange.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eProvides reliable and reproducible protein desalting and buffer exchanges of samples up to 15 mL.\u003c\/li\u003e\n\u003cli\u003eTwo to four columns can be connected in series for sample sizes from 30 to 60 mL.\u003c\/li\u003e\n\u003cli\u003eReplaces dialysis for desalting protein, which saves time and reduces labor.\u003c\/li\u003e\n\u003cli\u003eHiPrep format compatible with peristaltic pumps or chromatography systems such as ÄKTA systems.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eColumn for desalting and buffer exchange of proteins\u003c\/p\u003e\u003cp\u003eHiPrep 26\/10 Desalting is a prepacked, ready-to-use column for group separation between high and low molecular weight substances. Applications include buffer exchange prior to or after different chromatographic steps, removal of low molecular weight contaminants, or removal of reagents to terminate a reaction.\u003c\/p\u003e\u003cp\u003eThe exclusion limit is approximately Mr 5000. This ensures group separation of proteins\/peptides larger than M\u003csub\u003er\u003c\/sub\u003e 5000 from smaller molecules.\u003c\/p\u003e\u003cp\u003eSuccessful use in published research\u003c\/p\u003e\u003cp\u003eHiPrep 26\/10 Desalting columns are used in a variety of research applications, including desalting in the purification process of myofilament proteins using a cleavable His6-tag.\u003c\/p\u003e\u003cp\u003eOther Cytiva products for buffer exchange or desalting\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cul\u003e\u003cil\u003ePD SpinTrap G-25 spin column for small sample quantities using a benchtop centrifuge – up to 180 µL sample volume\u003cil\u003e\u003cil\u003ePD MiniTrap G-25 gravity column for sample volumes up to 0.5 mL\u003c\/il\u003e\u003cil\u003ePD MidiTrap G-25 gravity column for sample volumes up to 1 mL\u003c\/il\u003e\u003cil\u003ePD-10 Desalting gravity column for sample volumes up to 2.5 mL\u003c\/il\u003e\u003cil\u003eHiTrap Desalting column for use with a syringe, a peristaltic pump, or a chromatography system\u003c\/il\u003e\u003cil\u003eDifferent types of Sephadex resins are also available as bulk resins\u003c\/il\u003e\u003c\/il\u003e\u003c\/il\u003e\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-12822-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-13805-pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"1","offer_id":45982029381711,"sku":"17508701","price":3372.0,"currency_code":"ILS","in_stock":true},{"title":"4","offer_id":45982029414479,"sku":"17508702","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-9531-original-png_f563412f-589a-4fe2-8f75-bceffca3f1db.png?v=1785957235","url":"https:\/\/www.danyel.co.il\/products\/hiprep-desalting-columns-with-sephadex-g-25-resin-17508702","provider":"danyel biotech","version":"1.0","type":"link"}