{"product_id":"hi-control-bl21-de3-chemically-competent-cells-60435-1","title":"HI-Control BL21(DE3) Chemically Competent Cells","description":"\u003cp\u003eThe HI-Control™ strains are based on E. cloni 10G ( DH10B) and BL21(DE3) strains. These strains harbour a plasmid bearing a constitutive lacI repressor gene, which provides tight control over promoters containing the lacO operator.\u003c\/p\u003e\u003cp\u003eTightly Control Protein Expression\t \t \u003cbr\u003e \tT7 Promoters\tNon-T7 lac promoters\u003cbr\u003e(T5-lac, tac, trc, lac)\tClone with low background target expression\tTransformation efficiencies\u003cbr\u003eHI-Control\u003cbr\u003eBL21(DE3)\t \t \t \t≥ 1×107 cfu\/µg\u003cbr\u003eHI-Control\u003cbr\u003e10G\t \t \t \t≥ 1×109 cfu\/µg\u003cbr\u003eGenotypes:\u003c\/p\u003e\u003cp\u003eHI-Control BL21 (DE3): F- ompT hsdSB (rB- mB-) gal dcm (DE3) Mini-F lacIq1(GentR)\u003c\/p\u003e\u003cp\u003eHI-Control 10G: mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 ɸ80dlacZΔM15 ΔlacX74 araD139 Δ (ara,leu)7697 galU galK rpsL (StrR) nupG λ− tonA Mini-F lacIq1 (GentR)\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eReduce leaky expression: The LacI repressor protein binds to the lacO operator, blocking transcription of downstream genes. In the absence of an induction agent such as lactose sugar or IPTG, target protein expression from the adjacent promoter is greatly reduced.\u003c\/li\u003e\n\u003cli\u003eObtain high protein yields:Both 10G and BL21(DE3)-based strains express high levels of protein. For expression from a T7 promoter, select the HI-Control BL21(DE3) strain. Other lac-based promoters like T5-lac, tac, trc, and lac, can be used in either HI-Control strain.\u003c\/li\u003e\n\u003cli\u003eAchieve high efficiency transformations: Highly efficient competent cells give excellent results from small amounts of plasmid DNA.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003ePlease note that the SUMO Protease and SUMO Fusion tag are modified from the original native proteins. Native or non-modified control proteins or proteases will not be compatible.\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eCompetent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_60435-1_SDS-60435-HI-Control-BL21DE3-Chemically-Competent-Cells\"\u003eSDS 60435 HI Control BL21DE3 Chemically Competent Cells\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_60435-1_SDS0025-60435-1-HI-Control-BL21-DE3-ChemComp\"\u003eSDS0025 60435 1 HI Control BL21 DE3 ChemComp\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_60435-1_HI-Control\"\u003eHI Control\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"Default Title","offer_id":45986433400911,"sku":"60435-1","price":1179.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/HI-Control-BL21_2528DE3_2529-Chemically-Competent-Cells_19a2333c-55d0-484c-b49e-1c365a2f061c.jpg?v=1785956144","url":"https:\/\/www.danyel.co.il\/products\/hi-control-bl21-de3-chemically-competent-cells-60435-1","provider":"danyel biotech","version":"1.0","type":"link"}