{"product_id":"heparin-sepharose-6-fast-flow-affinity-resin-17099825","title":"Heparin Sepharose™ 6 Fast Flow affinity resin","description":"\u003cp\u003eHeparin Sepharose 6 Fast Flow is an established BioProcess affinity resin for capture or intermediate purification of proteins with affinity for heparin.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eProven for industrial plasma protein purification of various proteins, including antithrombin III antibodies, and coagulation factors.\u003c\/li\u003e\n\u003cli\u003eBased on Sepharose Fast Flow resins, which are well established in industrial downstream processing. Hydrophilic base matrix ensures low levels of nonspecific binding and low levels of host cell-derived impurities in the elution pool.\u003c\/li\u003e\n\u003cli\u003eHeparin ligand provides enhanced coupling chemistry and chemically stable ligand attachment.\u003c\/li\u003e\n\u003cli\u003eFulfills industrial demands for security of supply, robust performance, and regulatory support.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eApplications of heparin affinity chromatography\u003c\/p\u003e\u003cp\u003eCytiva's heparin resin allows for fast and reliable separations of biomolecules with an affinity for heparin, including antithrombin III antibodies, coagulation factors, and other plasma proteins. DNA-binding proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors can also be purified.\u003c\/p\u003e\u003cp\u003eChemically stable heparin purification resin\u003c\/p\u003e\u003cp\u003eHeparin Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads, modified with sodium heparin. Immobilization is performed using chemically stable and oriented coupling, which delivers high binding capacity. The chemical stability of the heparin resin is solely dictated by the ligand.\u003c\/p\u003e\u003cp\u003eStreamlined Heparin Sepharose Fast Flow scale-up\u003c\/p\u003e\u003cp\u003eScale-up with this resin is straightforward and is further aided by a range of prepacked process development tools.\u003c\/p\u003e\u003cp\u003eSuccessful use in published research\u003c\/p\u003e\u003cp\u003eThis heparin resin is used in a variety of research applications, including:\n\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eStudy of the biological activity of recombinant \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC2551750\/\"\u003ehuman lactoferrin in glycoengineered Pichia pastoris\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eEvaluation of the function of the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC2667725\/\"\u003eMPH1 gene of saccharomyces cerevisiae\u003c\/a\u003e in Okazaki fragment processing\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eCompatible empty columns – research scale\u003c\/p\u003e\u003cp\u003eCytiva's solutions include a variety of empty columns. Heparin Sepharose 6 Fast Flow is compatible with gravity columns, Tricorn columns, XK columns and HiScale columns.\u003c\/p\u003e\u003cp\u003eCompatible empty columns – bioprocess scale\u003c\/p\u003e\u003cp\u003eFor process-scale applications, Cytiva recommends AxiChrom columns. Heparin Sepharose Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow.\u003c\/p\u003e\u003cp\u003ePrepacked Heparin Sepharose Fast Flow columns – research scale\u003c\/p\u003e\u003cp\u003eHeparin Sepharose Fast Flow resin is available in the following prepacked formats:\n\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eHiTrap Heparin Sepharose 6 FF: easy to use with a syringe, a peristaltic pump, or a chromatography system\u003c\/li\u003e\n\u003cli\u003eHiPrep Heparin FF: for convenient scale-up\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003ePrepacked Heparin Sepharose Fast Flow columns – bioprocess scale\u003c\/p\u003e\u003cp\u003ePrepacked ReadyToProcess columns are available on request. They eliminate the need for column packing and cleaning validation.\u003c\/p\u003e\u003cp\u003eOther Cytiva products for affinity purification of proteins\u003c\/p\u003e\u003cp\u003eLooking for a different purification solution? Browse Cytiva's resins and prepacked columns for affinity chromatography or let us help you find the right affinity product.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-11397-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-12494-pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"1 L","offer_id":45982014963791,"sku":"17099803","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"50 mL","offer_id":45982014996559,"sku":"17099801","price":4124.0,"currency_code":"ILS","in_stock":true},{"title":"250 mL","offer_id":45982015029327,"sku":"17099825","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Standard","offer_id":45982015062095,"sku":"17099804","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-8729-original-png_0cdbcc62-871a-4fb6-b56b-d88faaefec6b.png?v=1785956695","url":"https:\/\/www.danyel.co.il\/products\/heparin-sepharose-6-fast-flow-affinity-resin-17099825","provider":"danyel biotech","version":"1.0","type":"link"}