{"product_id":"hek-293-tet-off-advanced-cell-line-631152","title":"HEK 293 Tet-Off® Advanced Cell Line","description":"\u003cp\u003eHEK 293 Tet-Off Advanced is a transformed human embryonic kidney-derived cell line that expresses the tetracycline (Tet)-regulated transactivator Tet-Off Advanced. Inducible expression of any gene can be achieved by transfecting or transducing this cell line with a vector containing your gene of interest under the control of a tetracycline-responsive promoter. Expression is induced by the withdrawal of doxycycline (Dox) from the culture medium. Tet-inducible cell lines are shipped with Tet System Approved FBS and a control cell line created for inducible expression of luciferase.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eStably expressed Tet-Off transactivator protein in commonly used cell lines\u003c\/li\u003e\n\u003cli\u003eInduce expression of your GOI by removing Dox from the culture of transfected cells\u003c\/li\u003e\n\u003cli\u003eEasily create an inducible system by first placing your gene under control of a tetracycline-responsive promoter (e.g.,PTRE3G), transfecting it into one of these G418-resistant cell lines, and selecting for double-stable clones\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eInducible gene expression in mammalian cells\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/631122\/631122-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/Tet\/Tet%20Cell%20Lines%20Protocol-At-A-Glance.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Components%20List\/631152_071817.pdf\"\u003eHEK 293 Tet-Off Advanced Cell Line Components List\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRequired Products\u003c\/h2\u003e\n\u003cp\u003e⚠️ A Required Product (e.g. an index kit) must be purchased to use this product — order one for each unit you buy.\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eCat. #\u003c\/th\u003e\n\u003cth\u003eProduct\u003c\/th\u003e\n\u003cth\u003eSize\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e631101\u003c\/td\u003e\n\u003ctd\u003eTet System Approved FBS, US-Sourced\u003c\/td\u003e\n\u003ctd\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e631106\u003c\/td\u003e\n\u003ctd\u003eTet System Approved FBS\u003c\/td\u003e\n\u003ctd\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e","brand":"Takara Bio","offers":[{"title":"Each","offer_id":45986022817871,"sku":"631152","price":6024.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/1594.jpg?v=1782804815","url":"https:\/\/www.danyel.co.il\/products\/hek-293-tet-off-advanced-cell-line-631152","provider":"danyel biotech","version":"1.0","type":"link"}