{"product_id":"guide-it-mutation-detection-kit-631443","title":"Guide-it™ Mutation Detection Kit","description":"\u003cp\u003eThe Guide-it Mutation Detection Kit contains all the reagents needed for PCR-based identification of insertions or deletions generated during cellular non-homologous end joining (NHEJ) repair. The first step is the amplification of the putative target sequence directly from cells. This kit uses Terra Direct PCR Polymerase Mix and Buffer, so there is no need to extract genomic DNA from your cell population prior to amplification of your target sequence. The amplicon is then melted and hybridized to form the mismatched targets for cleavage by the Guide-it Resolvase. Sufficient material is provided for 100 amplification and cleavage reactions.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eSimple method to identify insertions or deletions in mammalian cells\u003c\/li\u003e\n\u003cli\u003eFaster and more efficient than the surveyor assay; amplify directly from your target cells\u003c\/li\u003e\n\u003cli\u003eComplete kit: Guide-it Resolvase, Terra Direct PCR Polymerase, and all buffers included\u003c\/li\u003e\n\u003cli\u003eValue: 100 reactions per kit; also available in a 25-reaction size for small-scale genome editing projects\u003c\/li\u003e\n\u003cli\u003ePerfect for confirming sequence changes downstream from Cas9\/CRISPR, TALENs, or zinc-finger nuclease studies\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eConfirmation of mutations caused by genome editing technologies, including CRISPR\/Cas9, TALENs, or zinc-finger nucleases\u003cbr\u003eDetecting insertions and deletions in mammalian genomic DNA\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/631443\/631443-631448.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/ST0287\/ST0287-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/Guide\/Guide-it%20Mutation%20Detection%20Kit%20Protocol-At-A-Glance_111616.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"100 Rxns","offer_id":45986026815567,"sku":"631443","price":3211.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/631443-Guide-it-Mutation-Detection-Kit-product-600.jpg?v=1785957509","url":"https:\/\/www.danyel.co.il\/products\/guide-it-mutation-detection-kit-631443","provider":"danyel biotech","version":"1.0","type":"link"}