{"product_id":"guide-it-crispr-genome-wide-sgrna-library-ngs-analysis-kit-632647","title":"Guide-it™ CRISPR Genome-Wide sgRNA Library NGS Analysis Kit","description":"\u003cp\u003eThe Guide-it CRISPR Genome-Wide sgRNA Library NGS Analysis Kit enables preparation of next-generation sequencing (NGS) libraries from cell populations screened using the Guide-it CRISPR Genome-Wide sgRNA Library System (Cat. # 632646), allowing for identification of sgRNA sequences and corresponding gene knockouts that have increased or decreased in frequency under the conditions of the screen. The kit provides all the\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eContains \u0026gt;76,000 sgRNAs based on the Brunello library which maximizes on-target specificity and activity, while minimizing off-target effects (download sgRNA representation data)\u003c\/li\u003e\n\u003cli\u003eEach gene in the human genome is targeted by four highly active sgRNAs, enabling screens with a single module\u003c\/li\u003e\n\u003cli\u003eModified sgRNA scaffold design for improved Cas9-sgRNA interaction and high editing efficiency\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003ePooled genome-wide phenotypic screens\u003cbr\u003eLoss-of-function genetic screens\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/ST1507\/ST1507-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/Guide\/Guide-it%20CRISPR%20Genome-Wide%20sgRNA%20Library%20System%20User%20Manual.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Components%20List\/632647-120717.pdf\"\u003eGuide-it CRISPR Genome-Wide sgRNA Library NGS Analysis Kit Components List\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"10 Rxns","offer_id":45986051915855,"sku":"632647","price":3756.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/632647-Guide-it-CRISPR-Genome-Wide-sgRNA-Library-NGS-Analysis-Kit-product-600.jpg?v=1782802680","url":"https:\/\/www.danyel.co.il\/products\/guide-it-crispr-genome-wide-sgrna-library-ngs-analysis-kit-632647","provider":"danyel biotech","version":"1.0","type":"link"}