{"product_id":"guide-it-crispr-cas9-system-green-632601","title":"Guide-it™ CRISPR\/Cas9 System (Green)","description":"\u003cp\u003eThe Guide-it CRISPR\/Cas9 System (Green) is a complete system for cloning and expression of a target single guide RNA (sgRNA) for mammalian gene modification studies using CRISPR\/Cas9 technology. The pGuide-it-ZsGreen1 Vector is used to simultaneously express Cas9, a target-specific sgRNA, and the exceptionally bright fluorescent protein ZsGreen1. The vector is prelinearized for simple insertion of a target sequence for sgRNA expression from the human U6 promoter; Cas9 nuclease and ZsGreen1 are coexpressed from a bidirectional CMV promoter. This kit also contains all of the\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eA single vector expresses your target-specific sgRNA from the human U6 promoter; Cas9 nuclease and a bright fluorescent marker (either ZsGreen1 or tdTomato) are expressed from a bidirectional CMV promoter\u003c\/li\u003e\n\u003cli\u003etdTomato and ZsGreen1 are exceptionally bright fluorescent proteins, 6x and 2.5x brighter than EGFP, respectively; these markers can be used to monitor transfection efficiency and\/or to enrich\/isolate transfected cells by flow cytometry\u003c\/li\u003e\n\u003cli\u003eSimple plasmid construction—design oligos specific to your target sequence and clone into the pre-linearized plasmid using the included ligation reagents and high-efficiency competent cells\u003c\/li\u003e\n\u003cli\u003eIncludes sufficient reagents for construction of 10 different target (sgRNA) expression plasmids\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eCloning and expression of a target sgRNA for mammalian genome editing using CRISPR\/Cas9 technology\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/ST0914\/ST0914-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/Guide\/Guide-it%20CRISPR-Cas9%20Systems%20User%20Manual_033015.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Components%20List\/632601-010715.pdf\"\u003eGuide-it CRISPR\/Cas9 System (Green) Components List\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pGuide-it-ZsGreen1%20%28Linear%29.gb\"\u003epGuide-it-ZsGreen1 (Linear) Vector Sequence in GenBank Format\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"1 System","offer_id":45986051096655,"sku":"632601","price":2425.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/632601-Guide-it-CRISPR-Cas9-System-Green-product-600.jpg?v=1782802654","url":"https:\/\/www.danyel.co.il\/products\/guide-it-crispr-cas9-system-green-632601","provider":"danyel biotech","version":"1.0","type":"link"}