{"product_id":"gt-t551-culture-medium-1l-bottle-wk551s","title":"GT-T551 Culture medium, 1L Bottle","description":"\u003cp\u003eGT-T551 is a culture medium ideally suited for expansion of human T lymphocytes (T cells). It is supplemented with human albumin (pharmaceutical grade), recombinant human insulin, L-glutamine, and streptomycin; no additional proteins or growth factors are added. The medium can support stable growth in the presence or absence of serum.\u003cbr\u003eAnti-CD3\/IL-2 T-cell activation can also be performed in this medium (Wang et al. 2013; Ai et al. 2014; Dodo et al. 2014). When using this activation method, the additional presence of RetroNectin enhances the proliferation of T cells (Yu et al. 2008; Ishikawa et al. 2014; Hosoi et al. 2014; Sakamoto et al. 2015; Li et al. 2015) and results in an expanded cell population that shows a higher proportion of naïve T cells than anti-CD3 antibody stimulation alone (Hosoi et al. 2014).\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eUsed for extended culture or expansion of T cells.\u003c\/li\u003e\n\u003cli\u003eSupplemented with human serum albumin, human insulin, L-glutamine, and streptomycin.\u003c\/li\u003e\n\u003cli\u003eEndotoxin tested (endotoxin \u0026lt;0.3 EU\/ml).\u003c\/li\u003e\n\u003cli\u003eCompatible with CultiLife Culture Bags for large-scale expansion of T cells.\u003c\/li\u003e\n\u003cli\u003eThe method of T-cell expansion by RetroNectin stimulation requires a license from Takara Bio Inc. for uses other than research.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eExpansion of T cells\u003cbr\u003eGene transfer by lentiviral or retroviral transduction\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/WK551S\/WK551S_AL4P005_y_COA.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD5663\/SD5663-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/WK551S\/WK551S_e.v1602.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"1,000 mL","offer_id":45986329165903,"sku":"WK551S","price":1078.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/GT-T551_bottle.jpg?v=1782808686","url":"https:\/\/www.danyel.co.il\/products\/gt-t551-culture-medium-1l-bottle-wk551s","provider":"danyel biotech","version":"1.0","type":"link"}