{"product_id":"glutamic-pyruvic-transaminase-enzyme-activity-tbp0070","title":"Glutamic Pyruvic Transaminase, Enzyme Activity","description":"\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProduct Details\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eForm:\u003c\/u\u003e Freeze-dried powder\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eSolubility:\u003c\/u\u003e Distilled water or dilute buffer\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eStability:\u003c\/u\u003e Store at -20° C (-4° F)\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eActivity:\u003c\/u\u003e 100 U\/mg\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eProtein:\u003c\/u\u003e 90%\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eContaminants:\u003c\/u\u003e Glyceraldehyde-3-P-dehydrogenase \u0026lt;0.001%;\u003c\/p\u003e\n\u003cp\u003eMalate dehydrogenase \u0026lt;0.001%;\u003c\/p\u003e\n\u003cp\u003eGlutamate oxaloacetate transaminase \u0026lt;0.001%\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eUnit Definition\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThat amount of enzyme which will catalyze the conversion of one micromole of a-ketoglutarate to L-glutamate, in potassium phosphate buffer, at pH 7.5 and 25°C.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eAssay Method\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe decrease in the absorbance at 340 nm, caused by the oxidation of NADH, is proportional to the catalytic activity of GPT.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eApplications\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e(L-alanine:2 oxoglutarate aminotransferase; EC 2.6.1.2) Glutamic pyruvic transaminase (GPT) is also known as alanine aminotransferase. It catalyzes the following reaction:\u003c\/p\u003e\n\u003cp\u003eGPT L-Alanine + a-Ketoglutarate ——\u0026gt; Pyruvate + L-Glutamate\u003c\/p\u003e\n\u003cp\u003eTransamination reactions play a significant role in intermediary metabolism. Transaminases require pyridoxal phosphate as a coenzyme for their catalytic activity. GPT is found in many animal and plant tissues and it’s activity is especially high in mammalian heart and liver. The enzyme exists in two distinct isoenzyme forms in mammalian tissues, the mitochondrial form and the cytoplasmic form. GPT from porcine heart has been extensively studied. It has a molecular weight of 100,000. Human GPT levels in serum are used in clinical diagnosis of liver and heart disease.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eReagents\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e1 M Potassium phosphate buffer, pH 7.5.\u003c\/li\u003e\n\u003cli\u003e15 M L-Alanine (103 mg\/ml) in buffer.\u003c\/li\u003e\n\u003cli\u003e31 M a-Ketoglutarate (45 mg\/ml) in buffer.\u003c\/li\u003e\n\u003cli\u003e008 M NADH disodium salt (5 mg\/ml) in distilled water. Prepare fresh.\u003c\/li\u003e\n\u003cli\u003eLactate dehydrogenase (LDH), 250 U\/ml in buffer. Prepare fresh immediately prior to assay.\u003c\/li\u003e\n\u003cli\u003eEnzyme (GPT) solution. Prepare in buffer to yield a final concentration of 0.1-0.2 U\/ml. Must be prepared fresh immediately prior to assay.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003c!--TBP0070-1KU TBP0070-5KU--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0070-Glutamic-Pyruvic-Transaminase-2.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0070_Glutamic-Pyruvic-Transaminase-Safety-Data-Sheet-1.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"1000 U","offer_id":45986499100751,"sku":"TBP0070-1000U","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"5000 U","offer_id":45986499133519,"sku":"TBP0070-5000U","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBP0070.jpg?v=1782812648","url":"https:\/\/www.danyel.co.il\/products\/glutamic-pyruvic-transaminase-enzyme-activity-tbp0070","provider":"danyel biotech","version":"1.0","type":"link"}