{"product_id":"glutamic-oxaloacetic-transaminase-got-aspartate-aminotransferase-ast-activity-colorimetric-assay-tbs","title":"Glutamic Oxaloacetic Transaminase (GOT)\/ Aspartate aminotransferase (AST) Activity Colorimetric Assay","description":"\u003cp\u003eAspartate Aminotransferase (EC2.6.1.1, AST), also known as glutamic-oxaloacetic transaminase (GOT), is an enzyme that catalyzes the reversible transfer of an amino group from aspartate. This reaction is a key part of the nitrogen metabolism and the citric acid cycle, connecting amino acid metabolism to energy production. AST is found in various tissues, particularly in liver (high concentration), heart (cardiac muscle), skeletal muscle, kidneys and brain. Cytoplasmic AST and mitochondrial AST are found in circulation. When elevated or reduced in the blood, it can indicate metabolic disorders. So it is a key marker in diagnosing or monitoring these conditions.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eTribio™ Aspartate Aminotransferase Activity Colorimetric Assay is based on a dehydrogenase coupled reaction converting chromophore to a colored formazan, which can be measured at OD 460 nm. The generated signal is proportional to the AST enzyme activity. The kit provides the easiest and most accurate approach to measuring AST activity from a variety of samples.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eSpecifically detect aspartate aminotransferase from cells and biological tissues.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eKit Contents\u003c\/strong\u003e\u003cstrong\u003e for 100 tests:\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 60%\" border=\"1px solid black\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"201\"\u003e\u003cstrong\u003eName\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"125\"\u003e\u003cstrong\u003ePart Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"201\"\u003eAssay Buffer\u003c\/td\u003e\n\u003ctd width=\"125\"\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"201\"\u003eEnzyme mix\u003c\/td\u003e\n\u003ctd width=\"125\"\u003e0.7 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"201\"\u003eEnzyme substrate I (9x)\u003c\/td\u003e\n\u003ctd width=\"125\"\u003e1.2 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"201\"\u003eEnzyme substrate II (20x)\u003c\/td\u003e\n\u003ctd width=\"125\"\u003e0.6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"201\"\u003eEnzyme substrate III (10x)\u003c\/td\u003e\n\u003ctd width=\"125\"\u003e1.1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"201\"\u003eDetection Probe (10x)\u003c\/td\u003e\n\u003ctd width=\"125\"\u003e1.1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"201\"\u003eStandard stock (10 mM)\u003c\/td\u003e\n\u003ctd width=\"125\"\u003e0.3 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"201\"\u003ePositive control (5x)\u003c\/td\u003e\n\u003ctd width=\"125\"\u003e70 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"201\"\u003eCo-factor (25x)\u003c\/td\u003e\n\u003ctd width=\"125\"\u003e0.5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eStorage conditions\u003c\/strong\u003e\u003cstrong\u003e: \u003c\/strong\u003eStore the Reagent at –20 ℃ for a year.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eTypical standard curve\u003c\/span\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe Typical Standard curve showed as an example in Fig.1. It can be used only for reference.\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-35882 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2023\/09\/TBS2013_01.png\" alt=\"\" width=\"545\" height=\"346\" data-lazy-sizes=\"auto, (max-width: 545px) 100vw, 545px\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eThe positive control graph is shown in Fig.2 as below.\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-35884 lazyloaded\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2023\/09\/TBS2013_02.png\" alt=\"\" width=\"516\" height=\"376\" data-lazy-sizes=\"auto, (max-width: 516px) 100vw, 516px\" data-was-processed=\"true\"\u003e\u003c!--TBS2013--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2026\/01\/TBS2013_Aspartate-Aminotransferase-Colorimetric-Assay_data_sheet_2026.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2026\/01\/TBS2013-GOT-AST-Activity-Colorimetric-Assay_MSDS_2024.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986420129871,"sku":"TBS2013","price":1776.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS2013.png?v=1782810742","url":"https:\/\/www.danyel.co.il\/products\/glutamic-oxaloacetic-transaminase-got-aspartate-aminotransferase-ast-activity-colorimetric-assay-tbs","provider":"danyel biotech","version":"1.0","type":"link"}