{"product_id":"glutamate-dehydrogenase-nad-dependent-enzyme-activity-tbp0024","title":"Glutamate dehydrogenase(NAD dependent), Enzyme Activity","description":"\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003ePreparation and Specification\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eAppearance:\u003c\/u\u003e White amorphous powder, lyophilized\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eActivity:\u003c\/u\u003e GradeⅡ 100 U\/mg-solid or more\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eContaminants:\u003c\/u\u003e NAD oxidase ≤1.0×10⁻²%\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProperties\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eStability:\u003c\/u\u003e Stable at -20°C for at least One year\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eMolecular weight:\u003c\/u\u003e approx. 260,000\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eIsoelectric point:\u003c\/u\u003e 5.6\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eMichaelis constants:\u003c\/u\u003e 9.21×10⁻³M (NH₃), 4.80×10⁻³M (α-Ketoglutarate)\u003c\/p\u003e\n\u003cp style=\"padding-left: 80px\"\u003e7.8×10⁻⁵M (L-Glutamate), 1.29×10⁻⁴M (NADH), 5.89×10⁻⁴M (NAD⁺)\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eStructure:\u003c\/u\u003e 6 subunits per enzyme molecule\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eInhibitors:\u003c\/u\u003e Heavy metals, PCMB, IAA\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eOptimum pH:\u003c\/u\u003e 7.5-8.0 (α-KG→L-Glu) 9.0 (L-Glu→α-KG)\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eOptimum temperature:\u003c\/u\u003e 55°C (α-KG→L-Glu) 50°C (L-Glu→α-KG)\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003epH Stability:\u003c\/u\u003e pH 5.0-10.0 (25°C, 20hr)\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eThermal stability:\u003c\/u\u003e below 50°C (pH 8.3, 10min)\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eApplications\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThis enzyme is useful for enzymatic determination of NH₃, α-ketoglutaric acid and L-glutamic acid, and for assay of leucine aminopeptidase and urease. This enzyme is also used for enzymatic determination of urea when coupled with urease in clinical analysis.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003c!--TBP0024-1KU TBP0024-5KU--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/02\/TBP0024-Glutamate-dehydrogenaseNAD-dependent-1.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/02\/TBP0024_Glutamate-dehydrogenaseNAD-dependent-Safety-Data-Sheet.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"1000U","offer_id":45986494349391,"sku":"TBP0024-1000U","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"5000U","offer_id":45986494382159,"sku":"TBP0024-5000U","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBP0024.png?v=1782812557","url":"https:\/\/www.danyel.co.il\/products\/glutamate-dehydrogenase-nad-dependent-enzyme-activity-tbp0024","provider":"danyel biotech","version":"1.0","type":"link"}