{"product_id":"glucose-6-phosphate-colorimetric-assay-tbs2103","title":"Glucose-6-Phosphate Colorimetric Assay","description":"\u003cp\u003eGlucose 6-Phosphate dehydrogenase (G6PDH) is a key regulatory enzyme in the first step of the pentose phosphate pathway. In the presence of NADP+ or NAD+, it oxidizes glucose-6-phosphate (G6P) to 6-Phosphogluconolactone (6PG) and NADPH or NADH. It plays a vital role in numerous biosynthetic pathways and is important for preventing oxidative damage. In humans, deficiency is an autosomal hereditary disorder that can result in the depletion of NADPH in red blood cells and increases the risk of hemolytic anemia in situations of oxidative stress. On the other hand, inhibition of G6PDH has been linked to anti-cancer activity by decreasing RNA biosynthesis and increasing the buildup of reactive oxygen species in tumor cells.\u003c\/p\u003e\n\u003cp\u003eTribioscience’s G6PDH Activity Colorimetric Assay kit provides a flexible, accurate, sensitive, and time-saving approach for detecting G6PDH activity in a wide variety of samples.\u003c\/p\u003e\n\u003cp\u003eIn this assay, G6PDH converts glucose-6-phosphate to 6-phosphogluconate and NAD(P)H, which in turn induces the Colorimetric Probe to generate an intense colored product at 460 nm as shown in Fig.1.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFig.1. Principle of the Assay\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-17804 lazyloaded\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2023\/07\/Picture1.png\" alt=\"\" width=\"522\" height=\"175\" data-was-processed=\"true\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlexible:\u003c\/strong\u003e Suitable for 96-well plate.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAccuracy:\u003c\/strong\u003e Absorbance measurement is proportional to the G6PDH concentration.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitive:\u003c\/strong\u003e Can detect as low as 0.2 mU\/mL.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTime-saving:\u003c\/strong\u003e Just incubate and read out style.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eMeasure G6PDH activity in a variety of samples like cells and tissues.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFig.2. Typical standard curve of NADH\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-17805 lazyloaded\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2023\/07\/Picture2.png\" alt=\"\" width=\"377\" height=\"272\" data-was-processed=\"true\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c!--TBS2103--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2023\/07\/TBS2103-G6P-colorimetric-assay-V01-2024.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2023\/07\/TBS2103-G6P-colorimetric-assay_MSDS_2024.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986451488847,"sku":"TBS2103","price":2097.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS2103.png?v=1782811261","url":"https:\/\/www.danyel.co.il\/products\/glucose-6-phosphate-colorimetric-assay-tbs2103","provider":"danyel biotech","version":"1.0","type":"link"}