{"product_id":"gesicle-producer-293t-cell-line-632617","title":"Gesicle Producer 293T Cell Line","description":"\u003cp\u003eThe Gesicle Producer 293T Cell Line is a subclone of the transformed human embryonic kidney cell line, HEK 293, which is highly transfectable and supports high levels of protein expression. The cell line also constitutively expresses the simian virus 40 (SV40) large T antigen. Gesicles are produced in these cells via co-overexpression of packaging mix\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eBroad tropism: Efficient delivery of active Cas9 protein and target-specific sgRNA to a broad range of cell types\u003c\/li\u003e\n\u003cli\u003eReduced off-target effects: Delivery of Cas9 protein eliminates genomic integration and reduces off-target effects\u003c\/li\u003e\n\u003cli\u003eGenome editing only when you want it: Tight control over dose and timing of delivery and editing\u003c\/li\u003e\n\u003cli\u003eThe Gesicle Producer 293T Cell Line is not provided with the system and may be purchased separately (Cat. # 632617)\u003c\/li\u003e\n\u003cli\u003eStem cell applications: We have demonstrated high-efficiency editing of endogenous genes in human iPS cells using a combination of CRISPR\/Cas9 gesicles and our DEF-CS culture system.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eCRISPR\/Cas9-mediated gene editing\u003cbr\u003eStem cell research\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/632617\/632617.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/632617\/632617-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/Gesicle%20Producer%20293T%20Cell%20Line%20Protocol\/Gesicle%20Producer%20293T%20Cell%20Line%20Protocol-at-a-Glance_121715.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"1 mL","offer_id":45986051326031,"sku":"632617","price":2532.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/6400_hr.jpg?v=1782802657","url":"https:\/\/www.danyel.co.il\/products\/gesicle-producer-293t-cell-line-632617","provider":"danyel biotech","version":"1.0","type":"link"}