{"product_id":"first-strand-cdna-synthesis-kit-27926101","title":"First-Strand cDNA Synthesis Kit","description":"\u003cp\u003eFirst-Strand cDNA Synthesis kits are designed to generate full-length first-strand cDNA from mRNA templates following upstream mRNA extraction, isolation, or purification.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eFor synthesis of single-stranded cDNA prior to amplification by PCR\u003c\/li\u003e\n\u003cli\u003ePreassembled bulk reaction mixes save time and minimize potential for pipetting errors\u003c\/li\u003e\n\u003cli\u003eAllows users to choose primers\u003c\/li\u003e\n\u003cli\u003eIncludes a choice of two primers: pd(N)6 random hexamers and NotI-(dT)18\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eFirst-Strand cDNA Synthesis Kit\u003c\/p\u003e\n\n\u003cp\u003eAfter first-strand cDNA synthesis, the sample can be used directly for \u003cem\u003ein vitro\u003c\/em\u003e amplification using PCR or for second-strand synthesis using the Gubler-Hoffman method.\u003c\/p\u003e\n\n\u003cp\u003ePrimers are not included in the preassembled First-Strand Reaction Mixes, thus allowing use of a primer of choice.\u003c\/p\u003e\n\n\u003cp\u003eTwo primers are included with the kit. pd(N)\u003csub\u003e6\u003c\/sub\u003e Primer (random hexamer) is used to prepare libraries for screening with antibodies, to increase the representation of 5'-ends of mRNAs having significant secondary structure, or to copy mRNAs lacking a poly(A) tail.\u003c\/p\u003e\n\n\u003cp\u003eThe NotI-(dT)18 Bifunctional Primer can be used to prime selectively on mRNA that has a poly(A) tail. It can also be used as a PCR primer following first-strand synthesis. The NotI restriction site, located at the 5'-end of the primer, is particularly useful for subsequent directional cloning of cDNA and PCR products.\u003c\/p\u003e\n\n\u003cp\u003eFirst-Strand cDNA Synthesis Kit contains the following reagents, sufficient for up to 55 first-strand syntheses, each of which will produce enough material for up to 15 amplification reactions: Bulk First-Strand cDNA Reaction Mixes, mRNA Standard, DTT Solution, RNase-Free Water, pd(N)\u003csub\u003e6\u003c\/sub\u003e Primer, NotI-(dT)18 Bifunctional Primer, and instruction booklet.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/27926101.pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"55 reactions","offer_id":45982298210383,"sku":"27926101","price":2737.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-31350-original-png_fcc183ed-2b7c-4d1e-839c-ec5783b85411.png?v=1785960177","url":"https:\/\/www.danyel.co.il\/products\/first-strand-cdna-synthesis-kit-27926101","provider":"danyel biotech","version":"1.0","type":"link"}