{"product_id":"fast-mouse-c3d-elisa-kit-tbs3022","title":"Fast Mouse C3d ELISA Kit","description":"\u003cp\u003eThe Fast Mouse C3d ELISA is a solid phase ELISA designed to measure mouse C3d levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated multiple steps in traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 3 hours, not need 4-5 hours (Fig. 1). The detection range is from 1 to 250 ng\/mL. The levels of mouse C3d samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural mouse C3d protein.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eTypical Data\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThis standard curve(R\u003csup\u003e2\u003c\/sup\u003e=0.998) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical data.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe minimum detectable dose (MOD) of mouse C3d is typically 1 ng\/ml.\u003c\/p\u003e\n\u003cp\u003eThe Intra-assay CV and the Inter-assay CV are \u0026lt; 10%.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSpecificity\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThis assay recognizes natural and recombinant mouse C3d.\u003c\/p\u003e\n\u003cp\u003eNo cross reaction: Human C3d.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSimple Procedures (Fig. 1)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-9528 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2022\/04\/TBS3022-1.png\" alt=\"\" width=\"609\" height=\"124\" data-lazy-sizes=\"auto, (max-width: 609px) 100vw, 609px\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSimple Standard Preparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone  wp-image-30145\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2022\/04\/TBS3022_01.png\" alt=\"\" width=\"683\" height=\"362\" data-lazy-sizes=\"auto, (max-width: 683px) 100vw, 683px\"\u003e\u003c\/p\u003e\n\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eAccurate Standard Curve\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-9530 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2022\/04\/TBS3022-3.png\" alt=\"\" width=\"405\" height=\"354\" data-lazy-sizes=\"auto, (max-width: 405px) 100vw, 405px\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eKit Content and Storage Conditions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"height: 333px\" border=\"1px solid black\" width=\"1183\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003ePart\u003c\/th\u003e\n\u003cth\u003ePart#\u003c\/th\u003e\n\u003cth\u003eDescription\u003c\/th\u003e\n\u003cth\u003eStorage of Opened\/Reconstituted\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eMouse C3d Microplate\u003c\/td\u003e\n\u003ctd\u003eTBS3022A\u003c\/td\u003e\n\u003ctd\u003e96 well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for mouse C3d.\u003c\/td\u003e\n\u003ctd\u003eThe unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMouse C3d Standard\u003c\/td\u003e\n\u003ctd\u003eTBS3022B\u003c\/td\u003e\n\u003ctd\u003e20 µl of Recombinant mouse C3d protein (10 µg\/mL).\u003c\/td\u003e\n\u003ctd\u003eAliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection A\u003c\/td\u003e\n\u003ctd\u003eTBS3022C\u003c\/td\u003e\n\u003ctd\u003e2.1 ml of mouse C3d antibody.\u003c\/td\u003e\n\u003ctd rowspan=\"6\"\u003eMay be stored for up to 3 months at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection B\u003c\/td\u003e\n\u003ctd\u003eTBS3022D\u003c\/td\u003e\n\u003ctd\u003e12 ml of Streptavidin-HRP.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAssay Diluent\u003c\/td\u003e\n\u003ctd\u003eTBS3022E\u003c\/td\u003e\n\u003ctd\u003e12 ml of a buffered protein base with preservatives.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10x Wash Buffer\u003c\/td\u003e\n\u003ctd\u003eTBS3000W\u003c\/td\u003e\n\u003ctd\u003e12 mL of concentrated solution (10x).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTMB Substrate\u003c\/td\u003e\n\u003ctd\u003eTBS3000T\u003c\/td\u003e\n\u003ctd\u003e12 mL of ultra-sensitive TMB substrate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd\u003eTBS3000S\u003c\/td\u003e\n\u003ctd\u003e6 mL of 2 N sulfuric acid.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eStore the unopened kit at 2-8 ℃. Do not use past kit expiration dates.\u003cbr\u003e\nThe kit contains sufficient materials to run an ELISA on one 96-well plate.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eRelated Products\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/tribo-mouse-il-1%ce%b2-elisa-kit\/\"\u003eTribo™ Mouse IL-1β ELISA Kit (TBS3030)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/tribo-mouse-il-4-elisa-kit\/\"\u003eTribo™ Mouse IL-4 ELISA Kit (TBS3032)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/tribo-mouse-il-6-elisa-kit\/\"\u003eTribo™ Mouse IL-6 ELISA Kit (TBS3040)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/tribo-mouse-il-10-elisa-kit\/\"\u003eTribo™ Mouse IL-10 ELISA Kit (TBS3044)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/tribo-mouse-il-12-p70-elisa-kit\/\"\u003eTribo™ Mouse IL-12 p70 ELISA Kit (TBS3047)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003c!--TBS3022--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2022\/04\/TBS3022_Mouse_C3d_elisa_v02_2024.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2022\/04\/TBS3022_Human_C3d_ELISA_MSDS-1.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986370846799,"sku":"TBS3022","price":1776.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS3022.jpg?v=1782809673","url":"https:\/\/www.danyel.co.il\/products\/fast-mouse-c3d-elisa-kit-tbs3022","provider":"danyel biotech","version":"1.0","type":"link"}