{"product_id":"fast-human-il-12-p70-elisa-tbs3204","title":"Fast Human IL-12 p70 ELISA","description":"\u003cp\u003eThe Fast Human IL-12 p70 ELISA is a solid phase ELISA designed to measure human IL-12 p70 levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1 hour, with no need for 5-6 hours (Fig. 1). The detection range is from 8 to 2000 pg\/mL. The levels of human IL-12 p70 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human IL-12 protein.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eTYPICAL DATA\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThis standard curve (R\u003csup\u003e2\u003c\/sup\u003e=0.998) is provided for demonstration only. A standard curve should be generated for each set of samples assayed.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSENSITIVITY\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe minimum detectable dose (MOD) of human IL-12 p70 is typically 6.5 pg\/ml.\u003c\/p\u003e\n\u003cp\u003eThe Intra-CV is \u0026lt; 10% and the Inter-CV is \u0026lt; 12%.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSPECIFICITY\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThis assay recognizes natural and recombinant human IL-12 P70.\u003c\/p\u003e\n\u003cp\u003eNo-cross reactivity: Human IL-12 p35; Human IL-12\/IL-23 p40; Human IL-23; Mouse IL-12 p70.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eSIMPLE PROCEDURES (Fig. 1)\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cimg class=\"alignnone wp-image-9217 lazyloaded\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/10\/TBS3204-3-1024x225.png\" alt=\"\" width=\"692\" height=\"152\" data-lazy-sizes=\"auto, (max-width: 692px) 100vw, 692px\" data-was-processed=\"true\"\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eSIMPLE STANDARD PREPARATION\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-11530 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/10\/TBS3204-5.png\" alt=\"\" width=\"691\" height=\"397\" data-lazy-sizes=\"auto, (max-width: 691px) 100vw, 691px\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eSTANDARD CURVE\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-9181 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/10\/P70.png\" alt=\"\" width=\"389\" height=\"321\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eKIT CONTENT AND STORAGE CONDITIONS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"height: 396px\" border=\"1px solid black\" width=\"1242\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"89\"\u003e\u003cstrong\u003ePART\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"66\"\u003e\u003cstrong\u003ePART#\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e\u003cstrong\u003eDESCRIPTION\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"274\"\u003e\n\u003cstrong\u003eSTORAGE OF \u003c\/strong\u003e\u003cstrong\u003eOPENED\/ \u003c\/strong\u003e\u003cstrong\u003eRECONSTITUTED\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"89\"\u003eHuman IL-12 Microplate\u003c\/td\u003e\n\u003ctd width=\"66\"\u003eTBS3204A\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e96-well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for human IL-12 p70.\u003c\/td\u003e\n\u003ctd width=\"274\"\u003eThe unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"89\"\u003eHuman IL-12 Standard\u003c\/td\u003e\n\u003ctd width=\"66\"\u003eTBS3204B\u003c\/td\u003e\n\u003ctd width=\"247\"\u003eRecombinant human IL-12 protein (Lyophilized).\u003cbr\u003e\nReconstitute to 0.5 mL Assay Buffer.\u003c\/td\u003e\n\u003ctd width=\"274\"\u003eStore at 2-8 °C for 6 months. After reconstituting in 0.5mL Assay Buffer, it can be stored at -20 ℃ for up to 1 month.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"89\"\u003eDetection A\u003c\/td\u003e\n\u003ctd width=\"66\"\u003eTBS3204C\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e2.1 mL of human IL-12 antibody.\u003c\/td\u003e\n\u003ctd rowspan=\"6\" width=\"274\"\u003eMay be stored for up to 6 months at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"89\"\u003eDetection B\u003c\/td\u003e\n\u003ctd width=\"66\"\u003eTBS3204D\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e0.25 mL of Streptavidin-HRP (1:50 Dilution).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"89\"\u003eAssay Diluent\u003c\/td\u003e\n\u003ctd width=\"66\"\u003eTBS3204E\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e25 mL of a buffered protein base with preservatives.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"89\"\u003e10x Wash Buffer\u003c\/td\u003e\n\u003ctd width=\"66\"\u003eTBS3000W\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e12 mL of concentrated solution (10x).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"89\"\u003eTMB Substrate\u003c\/td\u003e\n\u003ctd width=\"66\"\u003eTBS3000T\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e12 mL of ultra-sensitive TMB substrate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"89\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd width=\"66\"\u003eTBS3000S\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e6 mL of 2 N sulfuric acid.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eRELATIVE PRODUCTS\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/human-il-1-s-elisa-1-step-and-1hr\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eHuman IL-1ß ELISA (TBS3219)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/human-il-2-elisa-1-step-and-1hr\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eHuman IL-2 ELISA (TBS3220)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/human-il-4-elisa-1-step-and-1hr\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eHuman IL-4 ELISA (TBS3221)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/human-il-6-elisa-1-step-and-1hr\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eHuman IL-6 ELISA (TBS3223)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/human-il-7-elisa-1-step-and-1hr\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eHuman IL-7 ELISA (TBS3224)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/human-il-8-elisa-1-step-and-1hr\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eHuman IL-8 ELISA (TBS3225)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003c!--TBS3204--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/10\/TBS3204_human_IL12-p70_elisa_datasheet_V02.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/10\/TBS3204_Human-IL12P70ELISA_MSDS.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986374352975,"sku":"TBS3204","price":1913.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS3204_hp70.jpg?v=1782809777","url":"https:\/\/www.danyel.co.il\/products\/fast-human-il-12-p70-elisa-tbs3204","provider":"danyel biotech","version":"1.0","type":"link"}