{"product_id":"failsafe-pcr-system-pcramp-020","title":"FailSafe PCR System","description":"\u003cp\u003eThe FailSafe PCR System is the post optimization, bundled, and affordable kit that contains everything you need for successful PCR performance. The kit is multiplex PCR compatible, can amplify even the most difficult high-GC templates, and targets up to 20 kb in length.\u003c\/p\u003e\u003cp\u003eThe PCR system can be purchased after PCR conditions are optimised with the FailSafe PCR PreMix Selection kit, so you know which 2x Premix to choose for your template\/primer combination.\u003c\/p\u003e\u003cp\u003eChoose between 100, 250, or 1000 Enzyme Mix units, then combine with your top choice of 1, 2, or 8 out of the 12 PCR 2x PreMixes. The Enzyme Mix included has a fidelity of at least three times higher than Taq DNA polymerase.\u003c\/p\u003e\u003cp\u003eThe FailSafe 2x PreMixes and Enzyme Mix can be purchased with the PCR PreMix Selection Kit for initial optimization, with the PCR System, or, as individual components.\u003c\/p\u003e\u003cp\u003eApplications\u003c\/p\u003e\u003cp\u003eEnd point PCR applications\u003cbr\u003eCloning\u003cbr\u003eSequencing\u003cbr\u003eGene Expression\u003cbr\u003eMutation Analysis\u003c\/p\u003e\u003cp\u003eHow It Works\u003c\/p\u003e\u003cp\u003eStep 1\u003c\/p\u003e\u003cp\u003eStart with the FailSafe PCR PreMix Selection Kit to optimise end-point PCR conditions.\u003c\/p\u003e\u003cp\u003eStep 2\u003c\/p\u003e\u003cp\u003eAnalyse and determine which FailSafe PCR 2x PreMixes produce high fidelity results, based on the template\/primer pair combination.\u003c\/p\u003e\u003cp\u003eStep 3\u003c\/p\u003e\u003cp\u003ePurchase the post optimization FailSafe PCR System, which includes the Enzyme Mix plus your choice of 1, 2, or 8 out of the 12 PCR 2x PreMixes, OR, purchase the FailSafe PreMix and Enzyme components individually; and achieve reliable and consistent high-fidelity PCR results.\u003c\/p\u003e\u003cp\u003eFor a complete explanation of how FailSafe technology works, please read the manual.\u003c\/p\u003e\u003cp\u003eFigure 1. Amplification of an 80%-85% GC-rich region of the human fragile X gene. PCR was performed using the FailSafe PCR System, schematically depicted above. Lanes A-L show the amplification products resulting from PCR using the 12 FailSafe PCR PreMixes. Lane M, molecular weight marker. In this experiment, optimal amplification was obtained with FailSafe PCR PreMix J. The size of the expected amplicon is indicated by an arrow.\tFigure 2. Multiplex PCR of the human CFTR gene. The FailSafe PCR System amplified all five exons of the CFTR gene from as little as 1 ng of human genomic DNA.\u003cbr\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eBundled and affordable kit with the option to pick and choose your optimal FailSafe 2X PreMixes\u003c\/li\u003e\n\u003cli\u003eConsistent and high-fidelity PCR results\u003c\/li\u003e\n\u003cli\u003eExtremely high sensitivity and specificity\u003c\/li\u003e\n\u003cli\u003eFidelity at least 3x higher than Taq DNA polymerase\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eBefore purchasing, we strongly recommend first optimising your PCR conditions using the FailSafe PCR PreMix Selection Kit in order to know which 2X PreMixes to choose with your PCR System kit.\u003c\/p\u003e\u003cp\u003eIncludes FailSafe PCR Enzyme Mix, a blend of thermostable enzymes containing a 3′→5′ proofreading enzyme for high fidelity, plus your choice of 1, 2, or 8 of the FailSafe PCR 2X PreMixes (100 uL each). 2X PreMixes contain dNTPs, buffer, different concentrations of MgCl2, and PCR Enhancer with betaine.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/SDS_PCRAMP-020_sds-FailSafe-PCR-System\"\u003esds FailSafe PCR System\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_PCRAMP-020_FailSafe-PCR-Kits\"\u003eFailSafe PCR Kits\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_PCRAMP-020_Enzyme-Catalog-Full\"\u003eEnzyme Catalog Full\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_PCRAMP-020_F207-US-FailSafe\"\u003eF207 US FailSafe\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_FS99060_FailSafe-PCR-Kits\"\u003emanual\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"FS9901K","offer_id":45986441494607,"sku":"FS9901K","price":9055.0,"currency_code":"ILS","in_stock":true},{"title":"FS99100","offer_id":45986441527375,"sku":"FS99100","price":1112.0,"currency_code":"ILS","in_stock":true},{"title":"FS99250","offer_id":45986441560143,"sku":"FS99250","price":2523.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/FailSafe-PCR-System_bcd8cd1f-e856-428f-92b7-db31c7ac26f7.jpg?v=1785956264","url":"https:\/\/www.danyel.co.il\/products\/failsafe-pcr-system-pcramp-020","provider":"danyel biotech","version":"1.0","type":"link"}