{"product_id":"failsafe-pcr-premix-selection-kit-fs99060","title":"FailSafe PCR PreMix Selection Kit","description":"\u003cp\u003eThe PCR PreMix Selection Kit is ideal for optimising PCR conditions and performance. The kit is multiplex PCR compatible, can amplify even the most difficult high-GC templates, and targets up to 20 kb in length.\u003c\/p\u003e\u003cp\u003eOptimisation is done by adding a template, primers, and FailSafe PCR Enzyme Mix to each of the 12 FailSafe 2x PreMixes, followed by amplification. Results are analysed by agarose gel electrophoresis, which can indicate your best template\/primer pair combination based on the 12 individual FailSafe 2X PreMixes.\u003c\/p\u003e\u003cp\u003eOnce optimal PCR conditions are determined; consistent and high-fidelity PCR results can be achieved through replication with the same template\/primer and PreMix combination. The FailSafe PreMix Selection Kit is sufficient for 48 reactions (four optimization runs with each of the 12 total 2X PreMixes). Following optimization the FailSafe 2x PreMixes and Enzyme Mix can be purchased with the FailSafe PCR System as the bundled and affordable option, or, as individual components.\u003c\/p\u003e\u003cp\u003eApplications\u003c\/p\u003e\u003cp\u003eEnd point PCR applications\u003cbr\u003eCloning\u003cbr\u003eSequencing\u003cbr\u003eGene Expression\u003cbr\u003eMutation Analysis\u003c\/p\u003e\u003cp\u003eHow It Works\u003c\/p\u003e\u003cp\u003eStep 1\u003c\/p\u003e\u003cp\u003eStart with the FailSafe PCR PreMix Selection Kit to optimise end-point PCR conditions.\u003c\/p\u003e\u003cp\u003eStep 2\u003c\/p\u003e\u003cp\u003eAnalyse and determine which FailSafe PCR 2x PreMixes produce high-fidelity results, based on the template\/primer pair combination.\u003c\/p\u003e\u003cp\u003eStep 3\u003c\/p\u003e\u003cp\u003ePurchase the post optimization FailSafe PCR System, which includes the Enzyme Mix plus your choice of 1, 2, or 8 out of the 12 PCR 2x PreMixes, OR, purchase the FailSafe PreMix and Enzyme components individually; and achieve reliable and consistent high-fidelity PCR results.\u003c\/p\u003e\u003cp\u003eFor a complete explanation of how FailSafe technology works, please read the manual.\u003c\/p\u003e\u003cp\u003eFigure 1. Amplification of an 80%-85% GC-rich region of the human fragile X gene. PCR was performed using the FailSafe™ PCR System, schematically depicted above. Lanes A-L show the amplification products resulting from PCR using the 12 FailSafe PCR PreMixes. Lane M, molecular weight marker. In this experiment, optimal amplification was obtained with FailSafe PCR PreMix J. The size of the expected amplicon is indicated by an arrow.\tFigure 2. Multiplex PCR of the human CFTR gene. The FailSafe™ PCR System amplified all five exons of the CFTR gene from as little as 1 ng of human genomic DNA. L\u003cbr\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003ePCR performance optimisation\u003c\/li\u003e\n\u003cli\u003eAmplification of high-GC templates and robust amplification of targets up to 20 kb in length\u003c\/li\u003e\n\u003cli\u003eMultiplex PCR compatible\u003c\/li\u003e\n\u003cli\u003eFidelity at least 3x higher than Taq DNA polymerase.\u003c\/li\u003e\n\u003cli\u003eExtremely high sensitivity and specificity\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eThe PCR PreMix Selection Kit includes the FailSafe PCR Enzyme Mix (24 uL), a blend of thermostable enzymes containing a 3′→5′ proofreading enzyme for high fidelity, and all twelve of the FailSafe PCR 2X PreMixes (100 uL each). 2X PreMixes contain dNTPs, buffer, different concentrations of MgCl2, and PCR Enhancer with betaine.\u003c\/p\u003e\u003cp\u003eSimply add template, primers, and FailSafe PCR Enzyme Mix to each of the PreMixes and amplify to determine your optimal PCR conditions.\u003c\/p\u003e\n\u003ch2\u003eFAQs\u003c\/h2\u003e\n\u003cp\u003eFAQs\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/SDS_FS99060_sds-FailSafe-PCR-System\"\u003esds FailSafe PCR System\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_FS99060_FailSafe-PCR-Kits\"\u003eFailSafe PCR Kits\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_FS99060_Enzyme-Catalog-Full\"\u003eEnzyme Catalog Full\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_FS99060_F207-US-FailSafe\"\u003eF207 US FailSafe\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"Default Title","offer_id":45986441723983,"sku":"FS99060","price":891.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/FailSafe-PCR-PreMix-Selection-Kit_a20d2c23-4a81-4b96-9434-6e28a91d9821.jpg?v=1785956273","url":"https:\/\/www.danyel.co.il\/products\/failsafe-pcr-premix-selection-kit-fs99060","provider":"danyel biotech","version":"1.0","type":"link"}