{"product_id":"ez-tn5-transposase-tnp92110","title":"EZ-Tn5 Transposase","description":"\u003cp\u003eEZ-Tn5™ Transposase is a hyperactive form of Tn5 transposase. 1 The highly purified, single-subunit enzyme can be used to randomly insert (transpose or \"hop\") any EZ-Tn5 Transposon into any target DNA in vitro with an efficiency up to \u0026gt;10 6 insertion clones per standard reaction. When incubated with an EZ-Tn5 Transposon in the absence of Mg 2+, a stable EZ-Tn5 Transposome™ complex is formed. The Transposome is so stable that it can be electroporated into living cells. Once in the cell, the Transposome is activated by intracellular Mg 2+ and the EZ-Tn5 Transposon component is randomly inserted into the host's genomic DNA. The three-dimensional structure of a Transposome complex has been elucidated. 2\u003c\/p\u003e\u003cp\u003eApplications\u003c\/p\u003e\u003cp\u003eIn vitro insertion of an EZ-Tn5 Transposon into DNA cloned in vectors, such as plasmids, fosmids, cosmids, or BACs as well as in vitro insertion into linear DNA.*\u003cbr\u003ePreparation of EZ-Tn5 Transposomes for in vivo transposition following electroporation into living cells.**\u003c\/p\u003e\u003cp\u003eA typical EZ-Tn5 transposition reaction requires four components: (1) the EZ-Tn5 Transposase; (2) an EZ-Tn5 Transposon; (3) a target DNA; and (4) the presence of Mg 2+. The highly random insertion of an EZ-Tn5 Transposon into the target DNA 3 proceeds by a cut-and-paste mechanism, 1 catalysed by the EZ-Tn5 Transposase, and results in a 9-bp duplication of target DNA sequence immediately adjacent to both ends of the Transposon.\u003c\/p\u003e\u003cp\u003eBenefits\u003c\/p\u003e\u003cp\u003eSpecifically and uniquely recognises the Outer End sequences of naturally occurring Tn5 and mini-Tn5 transposons and the hyperactive Mosaic Ends of EZ-Tn5 Transposons.\u003c\/p\u003e\u003cp\u003eReferences\u003c\/p\u003e\u003cp\u003eGoryshin, I.Y. and Reznikoff , W.S. (1998) J. Biol. Chem. 273, 7367.\u003cbr\u003eDavies, D.R. et al. (2000) Science 289, 77.\u003cbr\u003eShevchenko, Y. et al. (2002) Nucleic Acids Res. 30, 2469.\u003c\/p\u003e\u003cp\u003e*Covered byissued and\/or pending patents, exclusively licensed or assigned to Epicentre® (an Illumina® Company).\u003cbr\u003e**The use of Transposome™ complexes for in vivo insertion of a transposon, including, but not limited, to HyperMu™ and EZ-Tn5™ Transposome™ complexes, is covered by U.S. Patent No. 6,159,736 and related patents and patent applications, exclusively licensed to Epicentre® (an Illumina® Company).\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCreate custom transposomes for in vitro transposomics\u003c\/li\u003e\n\u003cli\u003eIntegrate selectable markers, promoters or replication origins into DNA in vitro with EZ-Tn5 insertion kits\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003e10 units of EZ-Tn5 transposase is enough enzyme for 10 standard reactions.\u003c\/p\u003e\u003cp\u003ePlease note: Illumina requires that a Transposome License Acknowledgement is signed prior to processing orders for EZ-Tn5 Transposase. Please complete this form before submitting your online order for this item. Commercial customers or customers interested in using this enzyme for commercial purchases must purchase a license from Illumina prior to purchase.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_TNP92110_EZ-Tn5-Transposase\"\u003eEZ Tn5 Transposase\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_TNP92110_Enzyme-Catalog-Full\"\u003eEnzyme Catalog Full\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_TNP92110_F800-US-Transposons\"\u003eF800 US Transposons\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"Default Title","offer_id":45986473574479,"sku":"TNP92110","price":3357.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/EZ-Tn5-Transposase_7465a4e6-dc65-4f54-9740-f9049ef940cb.jpg?v=1785956827","url":"https:\/\/www.danyel.co.il\/products\/ez-tn5-transposase-tnp92110","provider":"danyel biotech","version":"1.0","type":"link"}