{"product_id":"exonuclease-vii-en510250","title":"Exonuclease VII","description":"\u003cp\u003eExonuclease VII has high enzymatic specificity for ssDNA and exhibits both 5´ to 3´ and 3´ to 5´ exonuclease activities. It is useful for rapid removal of single-stranded oligonucleotide primers from a completed PCR when different primers are required for subsequent PCR amplifications. Exonuclease VII digestion of ssDNA occurs in the absence of magnesium. Exonuclease VII can be inactivated by heating at 95 °C for 10 minutes.\u003c\/p\u003e\u003cp\u003eReferences\u003c\/p\u003e\u003cp\u003eLi, H. et al. (1991) Nucleic Acids Res. 19, 3139.\u003cbr\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRemove single-stranded oligonucleotide primers after PCR\u003c\/li\u003e\n\u003cli\u003eMinimise the effect of primers left over from previous PCR amplifications\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eUnit Definition: One unit of Exonuclease VII catalyses the release of 1 nmol of acid-soluble nucleotides from activated heat denatured calf thymus DNA in 30 minutes at 37 °C under standard assay conditions.\u003c\/p\u003e\u003cp\u003eStorage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 0.1 M NaCl, and 0.1% Triton® X-100.\u003c\/p\u003e\u003cp\u003eQuality Control: Exonuclease VII is tested for activity in degradation of ssDNA and is free of detectable RNase, endonuclease, and double-stranded exonuclease activities.\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eIncludes 5X Reaction Buffer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/SDS_EN510250_Exonuclease-VII\"\u003eExonuclease VII\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_EN510250_Exonuclease-VII\"\u003eExonuclease VII\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_EN510250_Reagent-components-Exonuclease-VII-Manual-GEN1242SW0326\"\u003eReagent components Exonuclease VII Manual GEN1242SW0326\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_EN510250_exonuclease-vii-e-coli\"\u003eexonuclease vii e coli\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_EN510250_Enzyme-Catalog-Full\"\u003eEnzyme Catalog Full\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"Default Title","offer_id":45986441003087,"sku":"EN510250","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/Exonuclease-VII_10a5ce82-eccf-48e2-9969-00bedc056839.jpg?v=1785956249","url":"https:\/\/www.danyel.co.il\/products\/exonuclease-vii-en510250","provider":"danyel biotech","version":"1.0","type":"link"}