{"product_id":"exonuclease-iii-ex4425k","title":"Exonuclease III","description":"\u003cp\u003eExonuclease III digests duplex DNA in a 3´ to ;5´ direction from a nick, a blunt end, or 3´-recessed end, producing stretches of ssDNA on the opposite strand. 5,6 Under defined reaction conditions, DNA degradation by Exonuclease III proceeds at a uniform rate yielding predictable and reproducible digestion results.\u003c\/p\u003e\u003cp\u003eApplications\u003c\/p\u003e\u003cp\u003eProduction of intermediates for site-directed mutagenesis. 1-3\u003cbr\u003eProduction of strand-specific radiolabelled probes. 4\u003c\/p\u003e\u003cp\u003eBecause the rate of exonucleolytic excision of deoxyribonucleotides by Exonuclease III is dependent upon reaction factors including temperature, ionic strength, template sequence, and enzyme-to-DNA ratios, 4,7 each template must be optimised using sample digestions to achieve the desired excision rate.\u003c\/p\u003e\u003cp\u003eExonuclease III is not active on 3´-protruding ends of four bases or more in length, ssDNA, or on thioester-linked nucleotides. 2 The enzyme also has intrinsic RNase H, 3´-DNA phosphatase, and apurinic DNA endonuclease activities. 1,6\u003c\/p\u003e\u003cp\u003eReferences\u003c\/p\u003e\u003cp\u003eSambrook, J. et al. (1989) in: Molecular Cloning: A Laboratory Manual (2nd ed.), Cold Spring Harbor Laboratory Press, New York.\u003cbr\u003eVandeyar, M.A. (1988) Gene 65, 129.\u003cbr\u003eLuckow, B. et al. (1987) Nucleic Acids Res. 15, 417.\u003cbr\u003eRichardson, C.C. et al. (1964) J. Biol. Chem. 239, 251.\u003cbr\u003eWeiss, B. (1976) J. Biol. Chem. 251, 1896.\u003cbr\u003eRogers, S.G. and Weiss, B. (1980) Meth. Enzymol. 65, 201.\u003cbr\u003eGuo, L.H. and Wu, R. (1982) Nucleic Acids Res. 10, 2065.\u003cbr\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eProduce stretches of ssDNA in dsDNA templates containing nicks, blunt ends or recessed 3' ends\u003c\/li\u003e\n\u003cli\u003eProtect protruding 3' ends because this exonuclease will not start digestion at protruding 3' overhangs\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eUnit Definition: One unit of Exonuclease III catalyses the release of 1 nmol of acid-soluble nucleotides from double stranded calf thymus DNA in 30 minutes at 37 °C under standard assay conditions.\u003c\/p\u003e\u003cp\u003eStorage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton® X-100.\u003c\/p\u003e\u003cp\u003eExonuclease III 10X Reaction Buffer: 330 mM Tris-acetate (pH 7.5), 660 mM potassium acetate, 100 mM magnesium acetate, and 5 mM DTT.\u003c\/p\u003e\u003cp\u003eQuality Control: Exonuclease III is free of detectable exogenous RNase, endonuclease, and single-stranded exonuclease activities.\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eThis product includes both Exonuclease III enzyme and a 10X reaction buffer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/SDS_EX4425K_Exonuclease-III-E-coli\"\u003eExonuclease III E coli\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_EX4425K_SDS071-30263-1-30263-2-NxGen-Exonuclease-III\"\u003eSDS071 30263 1 30263 2 NxGen Exonuclease III\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_EX4425K_MA054E-Exonuclease-III\"\u003eMA054E Exonuclease III\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_EX4425K_Enzyme-Catalog-Full\"\u003eEnzyme Catalog Full\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"Default Title","offer_id":45986441429071,"sku":"EX4425K","price":1598.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/Exonuclease-III_cc270b04-b635-4b56-b159-5a363d451e95.jpg?v=1785956261","url":"https:\/\/www.danyel.co.il\/products\/exonuclease-iii-ex4425k","provider":"danyel biotech","version":"1.0","type":"link"}