{"product_id":"exonuclease-i-e70073z","title":"Exonuclease I","description":"\u003cp\u003eExonuclease I is used to selectively digest excess single stranded DNA in a mixture also containing double-stranded DNA, such as removing unused primers after PCR.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e3'→5' single-stranded exonuclease.\u003c\/li\u003e\n\u003cli\u003eMonomeric 54.5 kDa bacterial exonuclease I recombinantly expressed in E. coli.\u003c\/li\u003e\n\u003cli\u003eInactivated by heating at 80°C for 15 minutes.\u003c\/li\u003e\n\u003cli\u003eActive in PCR buffers.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eExonuclease I, used together with \u003ca href=\"\/shop\/molecular-biology\/pcr-and-amplification\/gel-extraction-and-clean-up\/illustra-shrimp-alkaline-phosphatase-p-00388\"\u003eAlkaline Phosphatase\u003c\/a\u003e for inactivation of dNTPs, provides effective cleanup of PCR reactions for subsequent use in DNA sequencing.\u003c\/p\u003e\n\n\u003cp\u003eDigestion of single stranded DNA by Exonuclease I releases dNTPs leaving 5'-terminal dinucleotides intact. DNA strands with terminal 3'-OH groups blocked by phosphoryl or acetyl groups are not removed.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/29046268.pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"2500 units","offer_id":45982218911823,"sku":"E70073Z","price":2492.0,"currency_code":"ILS","in_stock":true},{"title":"5000 units","offer_id":45982218944591,"sku":"E70073X","price":4241.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-34528-original-png_b09b2e3f-d925-44d2-b18e-7a78fe2f04b5.png?v=1785959149","url":"https:\/\/www.danyel.co.il\/products\/exonuclease-i-e70073z","provider":"danyel biotech","version":"1.0","type":"link"}