{"product_id":"exonuclease-i-e-coli-x40520k","title":"Exonuclease I, E. coli","description":"\u003cp\u003eExonuclease I digests ssDNA in a 3´ to 5´ direction, 1-3 but does not digest dsDNA. Although it requires the presence of magnesium and a free 3´-hydroxyl terminus for activity, it is active under a wide variety of buffer conditions and can be added directly into most reaction mixes. Exonuclease I can be heat-inactivated by incubation at 80 °C for 15 minutes.\u003c\/p\u003e\u003cp\u003eApplications\u003c\/p\u003e\u003cp\u003eRemoval of residual ssDNA, including oligos, from reaction mixes.\u003cbr\u003eRemoval of ssDNA from nucleic acid mixtures.\u003c\/p\u003e\u003cp\u003eFigure 1. Specificity of Exonuclease I for ssDNA. 200 ng of EcoR I-linearized pUC19 dsDNA and 1 µg of a 100-mer ssDNA oligo were mixed in 1X TA Buffer and incubated at 37°C for 20 min in the absence or presence of 10 units of Exonuclease I. As seen on this 1% agarose gel, Exonuclease I completely digested the linear ssDNA oligo, but left the linearised plasmid dsDNA intact. Lane 1, size markers; Lane 2, minus Exo I; Lane 3, plus Exo I.\u003c\/p\u003e\u003cp\u003eReferences\u003c\/p\u003e\u003cp\u003eLehman, I.R. and Nussbaum, A.L. (1964) J. Biol. Chem. 239, 2628.\u003cbr\u003eKushner, S.R. et al. (1971) Proc. Natl. Acad. Sci. USA 68, 824.\u003cbr\u003eKushner, S.R. et al. (1972) Proc. Natl. Acad. Sci. USA 69, 1366.\u003cbr\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eSpecific: Processively digest ssDNA in a 3´ to 5´ direction without harming dsDNA - short 3´ overhangs in DNA are not digested\u003c\/li\u003e\n\u003cli\u003eFlexible: Active under a wide variety of buffer conditions and can be added directly into most reaction mixes\u003c\/li\u003e\n\u003cli\u003eHeat Inactivated: Destroy the enzyme activity by incubating at 80°C for 15 minutes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eUnit Definition: One unit of Exonuclease I catalyses the release of 10 nmol of acid-soluble nucleotides from heat-denatured calf thymus DNA in 30 minutes at 37 °C under standard assay conditions.\u003c\/p\u003e\u003cp\u003eStorage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton® X-100.\u003c\/p\u003e\u003cp\u003eQuality Control: Exonuclease I is tested in degradation of ssDNA and is free of detectable RNase, endonuclease, and double-stranded exonuclease activities.\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eExonuclease I is supplied as enzyme only with no reaction buffer included.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/SDS_X40520K_Exonuclease-I\"\u003eExonuclease I\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_X40520K_SDS070-30262-1-30262-2-NxGen-Exonuclease-I\"\u003eSDS070 30262 1 30262 2 NxGen Exonuclease I\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_X40520K_Exonuclease-I\"\u003eExonuclease I\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_X40520K_exonuclease-i-e-coli\"\u003eexonuclease i e coli\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_X40520K_Enzyme-Catalog-Full\"\u003eEnzyme Catalog Full\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"Default Title","offer_id":45986473705551,"sku":"X40520K","price":2214.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/Exonuclease-I_252C-E-coli_a10e091f-6211-4316-bbc3-a1312cf73d64.jpg?v=1785956835","url":"https:\/\/www.danyel.co.il\/products\/exonuclease-i-e-coli-x40520k","provider":"danyel biotech","version":"1.0","type":"link"}