{"product_id":"exo-minus-klenow-dna-polymerase-d355a-e357a-nxgenz-004","title":"Exo-Minus Klenow DNA Polymerase (D355A, E357A)","description":"\u003cp\u003eExo-Minus Klenow DNA Polymerase is a DNA-dependent DNA polymerase that lacks both the 5´ to 3´ and 3´ to 5´ exonuclease activities of E. coli DNA Polymerase I, from which it is derived. This N-terminal truncation of DNA Polymerase I also has two mutations (D355A and E357A).\u003c\/p\u003e\u003cp\u003eReferences\u003c\/p\u003e\u003cp\u003eFeinberg, A.P. and Vogelstein, B. (1984) Anal. Biochem. 137, 266.\u003cbr\u003eSanbrook, J. et al. (1989) In: Molecular Cloning: A Laboratory Manual (2nd ed.), Cold Spring Harbor Laboratory Press, New York. v.2, p. 8.14.\u003cbr\u003eWalter, N.G. (1995) J. Mol. Biol. 254, 856.\u003cbr\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eA Klenow DNA polymerase lacking all 5' to 3' and 3' to 5' exonuclease activity\u003c\/li\u003e\n\u003cli\u003eSynthesise DNA from ssDNA templates without degrading any single-stranded DNA\u003c\/li\u003e\n\u003cli\u003eSuitable for a variety of applications including random-primer labelling of DNA,1second-strand cDNA synthesis,2sStrand-displacement amplification3\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eThe Exo-Minus Klenow DNA Polymerase (D355A, E357A) Kits include Exo-Minus Klenow DNA Polymerase (D355A, E357A) at 10 U\/µL and 10X Reaction Buffer.\u003c\/p\u003e\u003cp\u003eThe \"enzyme only\" format contains Exo-Minus Klenow DNA Polymerase (D355A, E357A) at 10 U\/µL.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_NXGENZ-004_Exo-Minus-Klenow-DNA-Polymerase-D355A-E357A\"\u003eExo Minus Klenow DNA Polymerase D355A E357A\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_NXGENZ-004_Exo-Minus-Klenow\"\u003eExo Minus Klenow\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"E357A), 2 mL at 10,000 U\/mL, 20,000 U","offer_id":45986440577103,"sku":"E0141-10D3","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"E357A), 10 U\/µL 1,000 U","offer_id":45986440609871,"sku":"KL11101K","price":1735.0,"currency_code":"ILS","in_stock":true},{"title":"E357A) KIT, 10 U\/µL. 20000 U","offer_id":45986440642639,"sku":"KL11120K","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/Exo-Minus-Klenow-DNA-Polymerase-_2528D355A_252C-E357A_2529_2c7e8def-0ef4-4377-9a3c-7062bfaf16b6.jpg?v=1785956231","url":"https:\/\/www.danyel.co.il\/products\/exo-minus-klenow-dna-polymerase-d355a-e357a-nxgenz-004","provider":"danyel biotech","version":"1.0","type":"link"}