{"product_id":"episcript-rnase-h-reverse-transcriptase-kit-221","title":"EpiScript RNase H- Reverse Transcriptase Kit","description":"\u003cp\u003eEpiScript RNase H-- Reverse Transcriptase (EpiScript RT), is a recombinant MMLV reverse transcriptase with greatly reduced RNase H activity. It is highly efficient at producing full-length cDNA from long RNA templates. EpiScript RT is capable of producing cDNA from as little as 50 pg of total RNA for real-time RT-PCR (qRT-PCR) analysis and other applications.\u003c\/p\u003e\u003cp\u003eApplications\u003c\/p\u003e\u003cp\u003eFirst-strand cDNA synthesis for subsequent PCR or real-time PCR.\u003c\/p\u003e\u003cp\u003eBenefits\u003c\/p\u003e\u003cp\u003eRecombinant MMLV reverse transcriptase with greatly reduced RNase H activity\u003cbr\u003eActive at temperatures up to 55 °C\u003cbr\u003eHighly efficient at producing full-length cDNAs\u003cbr\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eProduce full-length cDNA with RNAse H-mutant\u003c\/li\u003e\n\u003cli\u003eActive at elevated temperatures up to 55 °C\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eStorage: Store only at -20 °C in a freezer without a defrost cycle.\u003c\/p\u003e\u003cp\u003eStorage Buffer: EpiScript RT is supplied in a 50% glycerol solution containing 50 mM Tris-HCl (pH 7.5), 100 mM sodium chloride, 1 mM DTT, 0.1 mM EDTA, and 0.1% Triton® X-100.\u003c\/p\u003e\u003cp\u003eUnit Definition: One unit of EpiScript RT catalyses the incorporation of 1 nmol of dTTP into acid-insoluble material in 10 minutes at 37 °C using saturating amounts of oligo(dT)-primed poly(rA) as template.\u003c\/p\u003e\u003cp\u003eContaminating Activity Assays: EpiScript RT is free of detectable exonuclease, endonuclease, and RNase activities.\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eKit Contents: EpiScript RNase H- Reverse Transcriptase (200 U\/µL), 10X Reaction Buffer, 100 mM DTT.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_221_ERT12925K-1-EpiScript-RNase-H-Reverse-Transcriptase-English-US\"\u003eERT12925K 1 EpiScript RNase H Reverse Transcriptase English US\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_221_ERT12925K-EpiScript-RNase-H-Reverse-Transcriptase\"\u003eERT12925K EpiScript RNase H Reverse Transcriptase\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_221_MA353E-EpiScript-RT\"\u003eMA353E EpiScript RT\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_221_Enzyme-Catalog-Full\"\u003eEnzyme Catalog Full\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"Default Title","offer_id":45986441035855,"sku":"ERT12910K","price":727.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/EpiScript-RNase-H--Reverse-Transcriptase-Kit_e0d1c598-c23b-45b2-8fa7-2383bf31f589.jpg?v=1785956249","url":"https:\/\/www.danyel.co.il\/products\/episcript-rnase-h-reverse-transcriptase-kit-221","provider":"danyel biotech","version":"1.0","type":"link"}