{"product_id":"episcope-unmethylated-hct116-dko-gdna-3521","title":"EpiScope® Unmethylated HCT116 DKO gDNA","description":"\u003cp\u003eThis product consists of genomic DNA purified from the DNMT DKO (double knock-out) human HCT116 cell line, which is genetically lacking of both methyltransferases DNMT1 (DNA methyltransferase 1) and DNMT3B (DNA methytransferase 3B). The methylation level of the genomic DNA from DNMT DKO HCT116 is extremely low (\u0026lt; 5%) compared with wild type genomic DNA. Therefore, this DNA can be used as a negative (non- or hypo-methylated) control in DNA methylation analysis experiments.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eBisulfite sequencing\u003c\/li\u003e\n\u003cli\u003eCombined Bisulfite Restriction Analysis (COBRA)\u003c\/li\u003e\n\u003cli\u003eMethylated CpG Island Recovery Assay (MIRA)\u003c\/li\u003e\n\u003cli\u003eMethylation-specific PCR (MSP)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eBisulfite sequencing\u003cbr\u003eCombined Bisulfite Restriction Analysis (COBRA)\u003cbr\u003eMethylated CpG Island Recovery Assay (MIRA)\u003cbr\u003eMethylation-specific PCR (MSP)\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3521\/3521.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0540\/SD0540-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"10 ug","offer_id":45986003124303,"sku":"3521","price":1544.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3521-EpiScope-Unmethylated-HCT116-DKO-gDNA-product-600.jpg?v=1782801532","url":"https:\/\/www.danyel.co.il\/products\/episcope-unmethylated-hct116-dko-gdna-3521","provider":"danyel biotech","version":"1.0","type":"link"}