{"product_id":"endotoxin-free-ultrapure-plasmid-dna-maxiprep-tbs6037-10","title":"Endotoxin-free Ultrapure Plasmid DNA Maxiprep","description":"\u003cp\u003eEndotoxin-Free Utrapure Plasmid Extraction Maxiprep is composed of macroporous silica–based ion-exchange resin with a high density of diethylaminoethyl (DEAE) group, purification is based on the interaction between negatively charged phosphates of nucleic acids and positively charged DEAE groups on the surface of the resin, which could efficiently exclude all impurity during the binding-washing-elution process and yield ultrapure plasmid DNA.\u003c\/p\u003e\n\u003cp\u003eOur powerful endotoxin-removal reagent EF Reagent can effectively remove lipopolysaccharides from bacterial cell lysates by phase partition without chilling step. Simply add the EF reagent into the clear cell lysate, and the lipopolysaccharide contaminants can be removed with the flow-through. The Kit System consistently produces endotoxin level less than 0.1 EU\/μg DNA of endotoxin-free plasmid DNA product.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"163\"\u003e\u003cstrong\u003eParameter\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"163\"\u003e\u003cstrong\u003eValue\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"163\"\u003eDNA Fragment length\u003c\/td\u003e\n\u003ctd width=\"163\"\u003e1.5-kbp ⁓ 150 kbp\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"163\"\u003eMaximal Capacity\u003c\/td\u003e\n\u003ctd width=\"163\"\u003e\u0026gt;500µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"163\"\u003eMaximal Recovery\u003c\/td\u003e\n\u003ctd width=\"163\"\u003e99%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"163\"\u003eMinimal Elution Volume\u003c\/td\u003e\n\u003ctd width=\"163\"\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"163\"\u003eRegular Sample Volume\u003c\/td\u003e\n\u003ctd width=\"163\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"163\"\u003eEndotoxin Level\u003c\/td\u003e\n\u003ctd width=\"163\"\u003eLess Than 0.1EU\/ μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eFeature\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eIon-exchange based columns for rapid and simple extraction.\u003c\/li\u003e\n\u003cli\u003eReproducible yield of up to 500 µg of endotoxin-free plasmid DNA.\u003c\/li\u003e\n\u003cli\u003eEffective endotoxin removal reagent.\u003c\/li\u003e\n\u003cli\u003eEndotoxin level less than 0.1 EU\/µg DNA in the final DNA product.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eTransfection of sensitive cells (mammalian primary\/suspension cells).\u003c\/li\u003e\n\u003cli\u003eGene silencing study.\u003c\/li\u003e\n\u003cli\u003eMicroinjection.\u003c\/li\u003e\n\u003cli\u003eNGS.\u003c\/li\u003e\n\u003cli\u003eGene editing.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2023\/09\/TBS6037_-endotoxin-free-Maxi-PasmidProtocol_V01_2023.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2023\/09\/TBS6037_-endotoxin-free-Maxi-PasmidProtocol_MSDS-2024.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"10 Samples","offer_id":45986490220623,"sku":"TBS6037-10","price":1913.0,"currency_code":"ILS","in_stock":true},{"title":"25 Samples","offer_id":45986490253391,"sku":"TBS6037-25","price":3142.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS6037-2.jpg?v=1782812408","url":"https:\/\/www.danyel.co.il\/products\/endotoxin-free-ultrapure-plasmid-dna-maxiprep-tbs6037-10","provider":"danyel biotech","version":"1.0","type":"link"}