{"product_id":"endonuclease-viii-ngs-010","title":"Endonuclease VIII","description":"\u003cp\u003eE.coli Endonuclease VIII is a bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities. The N-glycosylase activity releases damaged pyrimidines from double-stranded DNA generating an apurinic (AP) site. The AP lyase activity cleaves the DNA phosphodiester backbone at AP sites via β- and δ-elimination, creating a one nucleotide gap with 5′ and 3′ phosphate termini. Damaged bases recognised and removed by Endonuclease VIII include thymine glycol, uracil glycol, urea, 5,6-dihydroxythymine, 5-hydroxy-5-methylhydantoin, 5-hydroxy-6-hydrothymine, 5,6-dihydrouracil and alloxan.\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e• Removes oxidatively damaged pyrimidines from DNA\u003c\/li\u003e\n\u003cli\u003e• Has both β and δ lyase activity, whereas Endonuclease III only has β lyase activity\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eEndonuclease VIII Kit contains Endonuclease VIII enzyme (10 U\/µL) and 10X Reaction Buffer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_NGS-010_Endonuclease-VIII\"\u003eEndonuclease VIII\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_NGS-010_Endonuclease-VIII-Reaction-Buffer-10X\"\u003eEndonuclease VIII Reaction Buffer 10X\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_NGS-010_E.-coli-Endonuclease-VIII-Kit\"\u003eE. coli Endonuclease VIII Kit\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"000 Units at 10","offer_id":45986431565903,"sku":"30270","price":486.0,"currency_code":"ILS","in_stock":true},{"title":"000 Units at 1","offer_id":45986431598671,"sku":"30271","price":3729.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/Endonuclease-VIII_653da74f-dbec-49be-87ce-33c49ce8d3f5.jpg?v=1785956114","url":"https:\/\/www.danyel.co.il\/products\/endonuclease-viii-ngs-010","provider":"danyel biotech","version":"1.0","type":"link"}