{"product_id":"elastase-enzyme-activity-tbp0067","title":"Elastase, Enzyme Activity","description":"\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProduct Details\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eForm:\u003c\/u\u003e Freeze-dried powder\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eSolubility:\u003c\/u\u003e Distilled water or dilute buffer\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eStability:\u003c\/u\u003e Store at -20° C (-4° F)\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eActivity:\u003c\/u\u003e 10 U\/mg protein\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eProtein:\u003c\/u\u003e 90%\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eUnit Definition\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe amount of enzyme which hydrolyzes one micromole of SucAla3Na per minute at 25°C, pH 8.0.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eAssay Method\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe assay method is described by Feinstein, et al.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eApplications\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eElastase (EC 3.4.21.36) is a unique proteolytic enzyme in mammals because of its ability to hydrolyze the insoluble protein elastin. In addition, it also exhibits endopeptidase activity on other proteins. Elastase is produced in the pancreas and it also occurs in leukocytes and in the serum. It has been postulated that elastase activity may play role in the induction of atherosclerosis and acute hemorrhagic pancreatitis.\u003c\/p\u003e\n\u003cp\u003eElastase from porcine pancreas has been extensively studied. It has a molecular weight of 25,900. Elastase can be used for degradation of proteins and peptides.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eReagents\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e0.1 M Tris buffer, pH 8.0.\u003c\/li\u003e\n\u003cli\u003e0.0044 M SucAla3Na substrate dissolved in Tris buffer (2 mg\/ml).\u003c\/li\u003e\n\u003cli\u003e0.15 NaCl\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProcedure\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eSet spectrophotometer (equipped with a strip chart recorder and temperature control) at 410 nm and 25°C.\u003c\/li\u003e\n\u003cli\u003ePipette into a cuvette:\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp style=\"padding-left: 40px\"\u003eTris Buffer 2.7 ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003eDiluted elastase 0.1 ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003eMix and incubate in a shaker-bath at 37°C for 4-5 min.\u003c\/p\u003e\n\u003col start=\"3\"\u003e\n\u003cli\u003eTo test cuvette add 0.2 ml of substrate, mix and record increase in A410 for 5 minutes.\u003c\/li\u003e\n\u003cli\u003eCalculate (delta)A\/min from the linear protion standard curve.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003c!--TBP0067-1MG TBP0067-5MG--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0067-Elastase-1.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0067_Elastase-Safety-Data-Sheet-1.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"1 mg","offer_id":45986498740303,"sku":"TBP0067-1MG","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"5 mg","offer_id":45986498773071,"sku":"TBP0067-5MG","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBP0067.png?v=1782812640","url":"https:\/\/www.danyel.co.il\/products\/elastase-enzyme-activity-tbp0067","provider":"danyel biotech","version":"1.0","type":"link"}