{"product_id":"econotaq-dna-polymerase-pcramp-003","title":"EconoTaq DNA Polymerase","description":"\u003cp\u003eQC specifications for EconoTaq DNA Polymerase are rigorous:\u003c\/p\u003e\u003cp\u003eGreater than 99% pure by SDS gel electrophoresis.\u003cbr\u003eNo detectable DNA contamination as determined by PCR using generic primers.\u003cbr\u003eNo detectable endonuclease (nicking) activity. Incubation of 10 U of EconoTaq DNA Polymerase with 1 µg of supercoiled pBR322 DNA for 16 hours at 70 °C results in no detectable conversion to relaxed or linear forms by agarose gel electrophoresis.\u003cbr\u003eNo detectable exonuclease activity. Incubation of 10 U of EconoTaq DNA Polymerase with 1 µg of Hind III-cut lambda DNA for 16 hours at 70°C results in no smearing of bands on agarose gels.\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eChoice of reaction buffers, with or without MgCl2.\u003c\/li\u003e\n\u003cli\u003eNon-proofreading Polymerase\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eConcentration: 5 units\/µl. One unit catalyses the incorporation of 10 nmol of dNTP into acid-insoluble material in 30 minutes at 70°C in 50 mM Tris-HCl (pH 9.0), 50 mM NaCl, 5 mM MgCl2, 200 µM dGTP, dATP, dTTP dCTP (a mix of un-labelled and [33P] dCTP), 10 µg of activated calf thymus DNA, and 0.1 mg\/ml BSA.\u003c\/p\u003e\u003cp\u003eStorage Buffer: 10 mM Tris-HCl (pH 7.5), 100 mM KCl, 0.1% Triton X-100, 0.1 mM EDTA, 1mM DTT, and 50% glycerol.\u003c\/p\u003e\u003cp\u003e10X Reaction Buffer:100mM Tris-HCl (pH 9.0), 500 mM KCl, 1% Triton X-100, and with or without 15mM MgCl2.\u003c\/p\u003e\u003cp\u003ePCR Activity: EconoTaq DNA Polymerase is tested in DNA amplification using a variety of templates and primers.\u003c\/p\u003e\u003cp\u003eActivity Determination: One unit of EconoTaq DNA Polymerase catalyses the incorporation of 10 nmoles of dNTP into acid-insoluble material in 30 minutes at 70 °C in 50 mM Tris-HCl (pH 9.0), 50 mM NaCl, 5 mM MgCl2, 200 µM dGTP, dATP, dTTP, dCTP (a mix of un-labelled and [33P]dCTP), 10 µg Activated Calf Thymus DNA, and 0.1 mg\/mL BSA.\u003c\/p\u003e\u003cp\u003eAbsence of Endonuclease or Nicking Activity: Incubation of 10 U of EconoTaq DNA Polymerase with 1 µg of supercoiled pBR322 DNA for 16 hours at 70 °C results in no detectable conversion to relaxed or linear forms detectable by agarose gel electrophoresis.\u003c\/p\u003e\u003cp\u003eAbsence of Exonuclease Activity: Incubation of 10 U of EconoTaq DNA Polymerase with 1 µg of HindIII-cut lambda DNA for 16 hours at 70 °C resulted in no smearing of bands on agarose gels.\u003c\/p\u003e\u003cp\u003ePurity: EconoTaq DNA Polymerase is \u0026gt;99% pure as determined by SDS PAGE. There is no detectable DNA contamination.\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eEconoTaq DNA Polymerase is provided with a choice of 10X Reaction Buffer with Mg++ (“with Mg++”); or 10X Reaction Buffer without Mg++ and a separate tube of 25 mM MgCl2 (“separate Mg++”).\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-003_30031-1-EconoTaq-DNA-Polymerase-with-Mg++\"\u003e30031 1 EconoTaq DNA Polymerase with Mg++\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-003_30031-EconoTaq-DNA-Polymerase\"\u003e30031 EconoTaq DNA Polymerase\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-003_30032-3-EconoTaq-DNA-Polymerase-separate%20Mg++\"\u003e30032 3 EconoTaq DNA Polymerase separate Mg++\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-003_30032-EconoTaq-DNA-Polymerase\"\u003e30032 EconoTaq DNA Polymerase\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-003_SDS-30032-EconoTaq-DNA-Polymerasee\"\u003eSDS 30032 EconoTaq DNA Polymerasee\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_PCRAMP-003_EconoTaq\"\u003eEconoTaq\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_PCRAMP-003_EconoTaq-woMg\"\u003eEconoTaq woMg\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_PCRAMP-003_Enzyme-Catalog-Full\"\u003eEnzyme Catalog Full\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_PCRAMP-003_F201-US0617-EconoTaq\"\u003eF201 US0617 EconoTaq\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"5 U\/µL,  1,000 U, Buffer with Mg++","offer_id":45986429337679,"sku":"30031-1","price":784.0,"currency_code":"ILS","in_stock":true},{"title":"5 U\/µL,  1,000 U, Buffer without Mg++","offer_id":45986429370447,"sku":"30032-1","price":784.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/EconoTaq-DNA-Polymerase_bbe59935-f7f0-4abe-8999-132940b11d9c.jpg?v=1785956073","url":"https:\/\/www.danyel.co.il\/products\/econotaq-dna-polymerase-pcramp-003","provider":"danyel biotech","version":"1.0","type":"link"}