{"product_id":"e-z-n-a-total-rna-kit-i-r6834-00","title":"E.Z.N.A.® Total RNA Kit I","description":"\u003cdiv class=\"product-summary\"\u003e\u003cul\u003e \u003cli\u003eRapid – 20 minutes or less\u003c\/li\u003e \u003cli\u003eSafe – No phenol\/chloroform extractions\u003c\/li\u003e \u003cli\u003eVersatile – Spin and vacuum formats available\u003c\/li\u003e \u003cli\u003eHigh quality – Purified RNA suitable for a variety of downstream applications\u003c\/li\u003e \u003c\/ul\u003e\u003c\/div\u003e\n\u003cdiv\u003e \u003cdiv\u003e \u003cp\u003eThe E.Z.N.A. Total RNA Kit I provides a simple and rapid method for the isolation of up to 100 μg total RNA from cultured eukaryotic cells and soft tissues. Multiple samples of up to 1 x 107 eukaryotic cells or 30 mg of tissue can be processed in parallel in fewer than 20 minutes. Many different sample types can be used, including kidney, liver, heart, spleen, lung, pancreas, thymus, HeLa cells, HEK-293 cells, and HINT3T3 cells. After the homogenization process, samples are applied to the HiBind® RNA Mini Column which binds total RNA. Cellular debris and other contaminants are effectively washed away after a few quick wash steps. Total RNA greater than 200 nt is isolated using this kit. Purified RNA can be used in many downstream applications such as RT-PCR, qRT-PCR, Northern blotting, nuclease protection assay, microarrays, \u003cem\u003ein-vitro \u003c\/em\u003etranslation, and Next Generation Sequencing.\u003c\/p\u003e \u003c\/div\u003e \u003c\/div\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eDownstream Application\u003c\/th\u003e\n\u003ctd\u003ePCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eElution Volume\u003c\/th\u003e\n\u003ctd\u003e40-70 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStarting Material\u003c\/th\u003e\n\u003ctd\u003eCultured eukaryotic cells and soft tissues\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStarting Amount\u003c\/th\u003e\n\u003ctd\u003e\u0026lt; 1 x 107 cells or 30 mg tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eRNA Yield\u003c\/th\u003e\n\u003ctd\u003eUp to 100 µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProcessing Mode\u003c\/th\u003e\n\u003ctd\u003eManual, centrifugation\/vacuum\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eThroughput\u003c\/th\u003e\n\u003ctd\u003e1 – 24\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eRNA Binding Technology\u003c\/th\u003e\n\u003ctd\u003eSilica Mini spin column\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBinding Capacity\u003c\/th\u003e\n\u003ctd\u003e100 µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ensur.omegabio.com\/ensur\/contentAction.aspx?key=Production.4635.S2R4E1A3.20190820.67.4690928\"\u003eProtocol Manual\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ensur.omegabio.com\/ensur\/contentAction.aspx?key=Production.2008.S2R4E1A3.20230907.294.4909817\"\u003eQuick Guide\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ensur.omegabio.com\/ensur\/contentAction.aspx?key=Production.6840.S2R4E1A3.20210528.8.4454494\"\u003eSafety Data Sheet\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Omega Bio-tek","offers":[{"title":"5 Preps","offer_id":45981779329103,"sku":"R6834-00","price":97.0,"currency_code":"ILS","in_stock":true},{"title":"50 Preps","offer_id":45981779361871,"sku":"R6834-01","price":854.0,"currency_code":"ILS","in_stock":true},{"title":"200 Preps","offer_id":45981779394639,"sku":"R6834-02","price":2848.0,"currency_code":"ILS","in_stock":true},{"title":"600 Preps","offer_id":45981779427407,"sku":"R6834-03","price":7923.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/MiniSpinColumn-withCap-R6731-R6812-R6834-copy.webp?v=1782734956","url":"https:\/\/www.danyel.co.il\/products\/e-z-n-a-total-rna-kit-i-r6834-00","provider":"danyel biotech","version":"1.0","type":"link"}