{"product_id":"e-z-n-a-plasmid-dna-midi-kit-d6904-00","title":"E.Z.N.A.® Plasmid DNA Midi Kit","description":"\u003cdiv class=\"product-summary\"\u003e\u003cul\u003e \u003cli\u003eSpeed – Purification of plasmid DNA in \u0026lt; 60 minutes\u003c\/li\u003e \u003cli\u003eSafety – No phenol\/chloroform extractions\u003c\/li\u003e \u003cli\u003eVersatile – Spin and vacuum formats available\u003c\/li\u003e \u003cli\u003eHigh quality – DNA is suitable for a variety of downstream applications\u003c\/li\u003e \u003c\/ul\u003e\u003c\/div\u003e\n\u003cdiv\u003e \u003cdiv\u003e \u003cp\u003eThe E.Z.N.A. Plasmid Midi and \u003ca href=\"https:\/\/omegabiotek.com\/product\/plasmid-dna-extraction-e-z-n-a-plasmid-dna-maxi-kit\/\"\u003eM\u003cspan\u003eaxi Kits \u003c\/span\u003e\u003c\/a\u003e\u003cspan\u003ecan isolate up to 250 μg and 1.2 mg of plasmid DNA from 50 mL and 200 mL bacterial cultures, respectively. These kits use a modified alkaline lysis method to lyse the cells and separate genomic DNA from plasmid DNA. Cellular debris are removed by centrifugation, and the protocol follows a simple bind, wash, and elute procedure to deliver high-quality\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eplasmid DNA. The system not only eliminates the time-consuming isopropanol precipitation step required by gravity flow columns but also the need for expensive accessories. Purified plasmid DNA is suitable for automated fluorescent DNA sequencing (typical reads exceed 800 bp), restriction enzyme digestion, ligation, PCR, in-vitro transcription, transformation, and other applications.\u003c\/p\u003e \u003c\/div\u003e \u003c\/div\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eDownstream Application\u003c\/th\u003e\n\u003ctd\u003eCloning, sequencing, transformation, PCR, restriction digestion, ligation, in vitro transcription etc.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStarting material\u003c\/th\u003e\n\u003ctd\u003e15-50 mL LB culture; Optimal Cell Mass (OD600 x mL culture) is 80-100.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePlasmid type\u003c\/th\u003e\n\u003ctd\u003eHigh-copy, low-copy, cosmid DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProcessing mode\u003c\/th\u003e\n\u003ctd\u003eManual (centrifugation or vacuum)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eThroughput\u003c\/th\u003e\n\u003ctd\u003e1 – 24\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDNA binding technology\u003c\/th\u003e\n\u003ctd\u003eSilica Midi Spin Column\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eLysate clearance method\u003c\/th\u003e\n\u003ctd\u003eCentrifugation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProcessing time\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;60 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eYield\u003c\/th\u003e\n\u003ctd\u003e100-250 µg for high copy-number; 10-50 µg for low copy-number\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ensur.omegabio.com\/ensur\/contentAction.aspx?key=Production.3694.S2R4E1A3.20190208.67.4699519\"\u003eProtocol Manual\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ensur.omegabio.com\/ensur\/contentAction.aspx?key=Production.3641.S2R4E1A3.20230906.294.4910785\"\u003eQuick Guide\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ensur.omegabio.com\/ensur\/contentAction.aspx?key=Production.6782.S2R4E1A3.20210528.8.4463327\"\u003eSafety Data Sheet\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Omega Bio-tek","offers":[{"title":"2 Preps","offer_id":45981754589263,"sku":"D6904-00","price":111.0,"currency_code":"ILS","in_stock":true},{"title":"25 Preps","offer_id":45981754622031,"sku":"D6904-03","price":888.0,"currency_code":"ILS","in_stock":true},{"title":"100 Preps","offer_id":45981754654799,"sku":"D6904-04","price":3099.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/MidiColumn-D6904-copy.webp?v=1782734781","url":"https:\/\/www.danyel.co.il\/products\/e-z-n-a-plasmid-dna-midi-kit-d6904-00","provider":"danyel biotech","version":"1.0","type":"link"}