{"product_id":"e-z-n-a-plant-dna-ds-kit-d2411-00","title":"E.Z.N.A.® Plant DNA DS Kit","description":"\u003cdiv class=\"product-summary\"\u003e\n\u003cp\u003eIsolation of genomic DNA from plant samples with high amounts of polysaccharides \u0026amp; polyphenols.\u003c\/p\u003e \u003cul\u003e \u003cli\u003eRapid – Homogenizer Columns allow for faster processing\u003c\/li\u003e \u003cli\u003eVersatile – Reliable results from a variety of sample types\u003c\/li\u003e \u003cli\u003eSafe – No organic extractions\u003c\/li\u003e \u003cli\u003eHigh-quality – Purified DNA suitable for most applications\u003c\/li\u003e \u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e \u003cdiv\u003e \u003cp\u003eThe E.Z.N.A. Plant DNA DS Kit is designed for the efficient recovery of genomic DNA up to 30 kb in size from fresh, frozen or dried plant tissue samples rich in polysaccharides, polyphenols, or those with a lower DNA content. Up to 50 mg wet tissue (or 15 mg dry tissue) can be processed in less than 1 hour. These systems combine the reversible nucleic acid-binding properties of the matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction enzyme digestion and hybridization applications.\u003c\/p\u003e\n\u003cp\u003eThese procedures rely on the well-established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with the unique binding system to increase yields and provide high-quality DNA. The system eliminates the need for chloroform extractions traditionally associated with CTAB-based lysis methods. Samples are homogenized and lysed in a high salt buffer containing CTAB and with binding conditions optimized, DNA is purified using a DNA mini column. Salts, proteins, and other contaminants are removed to yield high-quality genomic DNA.\u003c\/p\u003e \u003c\/div\u003e \u003c\/div\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eStarting Amount\u003c\/th\u003e\n\u003ctd\u003eUp to 50 mg wet tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStarting Material\u003c\/th\u003e\n\u003ctd\u003eFresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols, or those having a lower DNA content\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eElution Volume\u003c\/th\u003e\n\u003ctd\u003e50-100 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTechnology\u003c\/th\u003e\n\u003ctd\u003eHiBind® DNA Mini Column\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProcessing Mode\u003c\/th\u003e\n\u003ctd\u003eManual\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eThroughput\u003c\/th\u003e\n\u003ctd\u003e1-24\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eNote\u003c\/th\u003e\n\u003ctd\u003eCTAB lysis\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ensur.omegabio.com\/ensur\/contentAction.aspx?key=Production.2165.S2R4E1A3.20190109.67.4687177\"\u003eProtocol Manual\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ensur.omegabio.com\/ensur\/contentAction.aspx?key=Production.9386.S2R4E1A3.20230906.294.4930629\"\u003eQuick Guide\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ensur.omegabio.com\/ensur\/contentAction.aspx?key=Production.6775.S2R4E1A3.20210528.8.4467078\"\u003eSafety Data Sheet\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Omega Bio-tek","offers":[{"title":"5 Preps","offer_id":45981752557647,"sku":"D2411-00","price":97.0,"currency_code":"ILS","in_stock":true},{"title":"50 Preps","offer_id":45981752590415,"sku":"D2411-01","price":854.0,"currency_code":"ILS","in_stock":true},{"title":"200 Preps","offer_id":45981752623183,"sku":"D2411-02","price":3675.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/MiniSpinColumn-withCap-D2411-D2485-R6827-R6840-copy.webp?v=1782734750","url":"https:\/\/www.danyel.co.il\/products\/e-z-n-a-plant-dna-ds-kit-d2411-00","provider":"danyel biotech","version":"1.0","type":"link"}