{"product_id":"e-z-n-a-hp-total-rna-kit-r6812-00","title":"E.Z.N.A.® HP Total RNA Kit","description":"\u003cdiv class=\"product-summary\"\u003e\u003cul\u003e \u003cli\u003eRapid – RNA isolation in 25 minutes\u003c\/li\u003e \u003cli\u003eVersatile – Spin and vacuum formats available\u003c\/li\u003e \u003cli\u003eSpecialized System – Sample Homogenization and gDNA elimination in one step\u003c\/li\u003e \u003cli\u003eSafe – No phenol\/chloroform extractions\u003c\/li\u003e \u003cli\u003eHigh-quality – RNA is suitable for a variety of downstream applications\u003c\/li\u003e \u003c\/ul\u003e\u003c\/div\u003e\n\u003cdiv\u003e \u003cdiv\u003e \u003cp\u003eThe E.Z.N.A.® HP Total RNA Isolation Kit provides a rapid and easy method for RNA isolation from cultured cells or tissues. The kit includes RNA Homogenizer columns that integrate sample homogenization and genomic DNA elimination into one single step. The RNA purification process is simplified with HiBind® Mini Column technology and can be accomplished in 25 minutes. This convenient spin-column format requires no phenol or chloroform, avoids time-consuming steps such as CsCl gradient ultracentrifugation, precipitation with isopropanol or LiCl and allows for multiple samples to be processed in parallel. RNA purified using the E.Z.N.A.® HP Total RNA method is ready for applications such as RT-PCR, RT-qPCR, Northern blotting, next-generation sequencing, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.\u003c\/p\u003e \u003c\/div\u003e \u003c\/div\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eDownstream Application\u003c\/th\u003e\n\u003ctd\u003eRT-PCR, RT-qPCR, Northern blotting, next-generation sequencing, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStarting Material\u003c\/th\u003e\n\u003ctd\u003eCultured cells and tissues\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStarting Amount\u003c\/th\u003e\n\u003ctd\u003e1 x 107 eukaryotic cells or 25-30 mg tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eElution Volume\u003c\/th\u003e\n\u003ctd\u003e40-70 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eRNA Binding Technology\u003c\/th\u003e\n\u003ctd\u003eSilica Mini spin column\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProcessing Mode\u003c\/th\u003e\n\u003ctd\u003eManual (centrifugation or vacuum)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eThroughput\u003c\/th\u003e\n\u003ctd\u003e1 – 24\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFinal Purified Product\u003c\/th\u003e\n\u003ctd\u003eTotal RNA (\u0026gt; 200 nt)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eRNA Yield\u003c\/th\u003e\n\u003ctd\u003eUp to 100 µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProcessing Time\u003c\/th\u003e\n\u003ctd\u003e25 min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecial Notes\u003c\/th\u003e\n\u003ctd\u003eRNA Homogenizer Column that integrates sample homogenization and gDNA elimination into a single step\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ensur.omegabio.com\/ensur\/contentAction.aspx?key=Production.4455.S2R4E1A3.20190820.67.4691170\"\u003eProtocol Manual\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ensur.omegabio.com\/ensur\/contentAction.aspx?key=Production.2049.S2R4E1A3.20230907.294.4918408\"\u003eQuick Guide\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ensur.omegabio.com\/ensur\/contentAction.aspx?key=Production.6781.S2R4E1A3.20210528.8.4461512\"\u003eSafety Data Sheet\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Omega Bio-tek","offers":[{"title":"5 Preps","offer_id":45981778837583,"sku":"R6812-00","price":117.0,"currency_code":"ILS","in_stock":true},{"title":"50 Preps","offer_id":45981778870351,"sku":"R6812-01","price":1095.0,"currency_code":"ILS","in_stock":true},{"title":"200 Preps","offer_id":45981778903119,"sku":"R6812-02","price":3621.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/MiniSpinColumn-withCap-R6731-R6812-R6834-copy.webp?v=1782734956","url":"https:\/\/www.danyel.co.il\/products\/e-z-n-a-hp-total-rna-kit-r6812-00","provider":"danyel biotech","version":"1.0","type":"link"}