{"product_id":"e-z-n-a-gel-extraction-kit-v-spin-d2500-00","title":"E.Z.N.A.® Gel Extraction Kit (V-spin)","description":"\u003cdiv class=\"product-summary\"\u003e\u003cul\u003e \u003cli\u003eRapid – DNA recovery from an agarose gel in 15 min\u003c\/li\u003e \u003cli\u003eSpecialized buffer system – Visual determination of optimum DNA binding for higher yields\u003c\/li\u003e \u003cli\u003eSafe – No phenol\/chloroform extractions\u003c\/li\u003e \u003cli\u003eVersatile – Spin and vacuum formats available\u003c\/li\u003e \u003cli\u003eHigh-quality – DNA is suitable for a variety of downstream applications\u003c\/li\u003e \u003c\/ul\u003e\u003c\/div\u003e\n\u003cdiv\u003e \u003cdiv\u003e \u003cp\u003eThe E.Z.N.A. Gel Extraction Kit uses spin-column technology to purify DNA fragments ranging from 100 bp to 10 kb from all grades of agarose gels with high recovery (\u0026gt; 80%). The kit uses a specialized binding buffer system that not only dissolves the gel slice and binds to the spin column but also includes a pH indicator for a visual representation of optimal pH for DNA binding. The bind step is followed by three rapid wash steps, and DNA is eluted with deionized water or elution buffer. Purified DNA is ready for a variety of downstream applications such as ligations, PCR amplification, restriction enzyme digestion, cloning, and various labeling reactions.\u003c\/p\u003e \u003c\/div\u003e \u003c\/div\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eDownstream application\u003c\/th\u003e\n\u003ctd\u003eCloning, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eElution volume\u003c\/th\u003e\n\u003ctd\u003e30-50 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStarting material\u003c\/th\u003e\n\u003ctd\u003eAgarose gel slice\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStarting amount\u003c\/th\u003e\n\u003ctd\u003eUp to 25 µg DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDNA recovered\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;85% recovery, 70 bp to 20 kb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProcessing mode\u003c\/th\u003e\n\u003ctd\u003eManual, centrifugation\/vacuum\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eThroughput\u003c\/th\u003e\n\u003ctd\u003e1-12\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDNA binding technology\u003c\/th\u003e\n\u003ctd\u003eSilica mini spin column\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBinding capacity\u003c\/th\u003e\n\u003ctd\u003e25 μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProcessing time\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;10 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ensur.omegabio.com\/ensur\/contentAction.aspx?key=Production.1999.S2R4E1A3.20181107.32.4668301\"\u003eProtocol Manual\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ensur.omegabio.com\/ensur\/contentAction.aspx?key=Production.3638.S2R4E1A3.20230906.294.4921796\"\u003eQuick Guide\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ensur.omegabio.com\/ensur\/contentAction.aspx?key=Production.6788.S2R4E1A3.20210528.8.4474217\"\u003eSafety Data Sheet\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Omega Bio-tek","offers":[{"title":"5 Preps","offer_id":45981752885327,"sku":"D2500-00","price":64.0,"currency_code":"ILS","in_stock":true},{"title":"50 Preps","offer_id":45981752918095,"sku":"D2500-01","price":345.0,"currency_code":"ILS","in_stock":true},{"title":"200 Preps","offer_id":45981752950863,"sku":"D2500-02","price":1196.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/MiniSpinColumn-withCap-D2500-copy.webp?v=1782734755","url":"https:\/\/www.danyel.co.il\/products\/e-z-n-a-gel-extraction-kit-v-spin-d2500-00","provider":"danyel biotech","version":"1.0","type":"link"}