{"product_id":"e-z-n-a-fastfilter-plasmid-dna-mini-kit-d6944-00","title":"E.Z.N.A.® FastFilter Plasmid DNA Mini Kit","description":"\u003cdiv class=\"product-summary\"\u003e\u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eRapid\u003c\/strong\u003e – 9 Minute Processing time from a bacterial pellet\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eUnique\u003c\/strong\u003e – Lysate Clearance and DNA Binding in 1 step\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eConvenient\u003c\/strong\u003e – Lysate Clearance Column eliminates cellular debris pelleting and transfer step\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eVersatile\u003c\/strong\u003e – Process up to 5 mL bacterial cultures with different plasmid types and culture medias\u003c\/li\u003e \u003c\/ul\u003e\u003c\/div\u003e\n\u003cdiv\u003e \u003cdiv\u003e \u003cp\u003eThe E.Z.N.A.® FastFilter Plasmid DNA Mini Kit is designed for rapid purification of high-quality plasmid DNA from up to 5 mL of culture following the alkaline-lysis method. The kit features an innovative and first-of-its-kind FastFilter Mini Column nested within a regular HiBind® DNA Mini Column to combine lysate clearance and DNA binding into one or two centrifugation steps depending on the culture volume. The protocol time is just 9 minutes when starting with bacterial pellet from up to 3 mL culture volume. The protocol time for culture volumes greater than 3 mL will be slightly longer due to two centrifugation steps for lysate clearance. Following the wash and elute steps, purified plasmid DNA is immediately ready for various downstream applications such as routine screening, restriction enzyme digestion, transformation, PCR, and DNA sequencing.\u003c\/p\u003e\n\u003cp\u003eTypically, up to 5 mL overnight bacterial culture in LB or other suitable growth medium is centrifuged to pellet the bacterial cells. The cells are then lysed under alkaline conditions and subsequently neutralized to form white flocculent precipitate. Neutralized lysate is then transferred to a FastFilter Mini Column nested within a HiBind® DNA Mini Column. Upon adjustment of binding conditions, the lysate is centrifuged through this FastFilter HiBind® configuration to both clear the lysate and facilitate binding of DNA to the HiBind® \u003cbr\u003eDNA Mini Column. The FastFilter Mini Column is discarded, and the HiBind® DNA Mini Column is subjected to two wash steps to remove salt and other contaminants. High-quality plasmid DNA is then eluted in low-salt elution buffer and is ready for use in a wide range of downstream applications mentioned above.\u003c\/p\u003e \u003c\/div\u003e \u003c\/div\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eStarting Material\u003c\/th\u003e\n\u003ctd\u003eBacteria harboring high-copy or low-copy plasmid in LB culture medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStarting Amount\u003c\/th\u003e\n\u003ctd\u003e1-5 mL LB culture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eElution Volume\u003c\/th\u003e\n\u003ctd\u003e50-100 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProcessing mode\u003c\/th\u003e\n\u003ctd\u003eManual (centrifugation or vacuum)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eThroughput\u003c\/th\u003e\n\u003ctd\u003e1-24\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDNA binding technology\u003c\/th\u003e\n\u003ctd\u003eSilica Mini Spin Column\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProcessing time\u003c\/th\u003e\n\u003ctd\u003e9 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDownstream Applications\u003c\/th\u003e\n\u003ctd\u003eRoutine screening, restriction enzyme digestion, transformation, PCR and DNA sequencing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ensur.omegabio.com\/ensur\/contentAction.aspx?key=Production.9248.S2R4E1A3.20220909.8.4478694\"\u003eProtocol Manual\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ensur.omegabio.com\/ensur\/contentAction.aspx?key=Production.6782.S2R4E1A3.20210528.8.4463327\"\u003eSafety Data Sheet\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Omega Bio-tek","offers":[{"title":"10 Preps","offer_id":45981755670607,"sku":"D6944-00","price":201.0,"currency_code":"ILS","in_stock":true},{"title":"100 Preps","offer_id":45981755703375,"sku":"D6944-01","price":690.0,"currency_code":"ILS","in_stock":true},{"title":"300 Preps","offer_id":45981755736143,"sku":"D6944-02","price":1769.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/MiniSpinColumn-D6944-copy.webp?v=1782734796","url":"https:\/\/www.danyel.co.il\/products\/e-z-n-a-fastfilter-plasmid-dna-mini-kit-d6944-00","provider":"danyel biotech","version":"1.0","type":"link"}