{"product_id":"e-z-n-a-fastfilter-plasmid-dna-midi-kit-d6905-00","title":"E.Z.N.A.® FastFilter Plasmid DNA Midi Kit","description":"\u003cdiv class=\"product-summary\"\u003e\u003cul\u003e \u003cli\u003eSpeed – Purification of plasmid DNA \u0026lt; 40 minutes\u003c\/li\u003e \u003cli\u003eSafety – No phenol\/chloroform extractions\u003c\/li\u003e \u003cli\u003eVersatile – Spin and vacuum formats available\u003c\/li\u003e \u003cli\u003eHigh quality – DNA is suitable for a variety of downstream applications\u003c\/li\u003e \u003c\/ul\u003e\u003c\/div\u003e\n\u003cdiv\u003e \u003cdiv\u003e \u003cp\u003eThe E.Z.N.A.® FastFilter Plasmid DNA Midi Kit combines the convenience of spin columns with the speed of syringe filters to ease large-scale plasmid preparations. The system utilizes Lysate Clearance Filter Syringes instead of centrifugation to rapidly clear bacterial lysates post-alkaline lysis and follows a simple “bind-wash-elute” procedure to deliver high quality plasmid DNA. The yields vary according to plasmid copy number, \u003cem\u003eE. coli\u003c\/em\u003e strain, and growth conditions. 15-50 mL of bacterial cultures in LB medium typically produces up to 200 µg of high copy number plasmid DNA. Up to 100 mL of culture may be processed when working with low copy number plasmid. The system uses centrifugation or vacuum technology for plasmid purification and eliminates the time-consuming gravity-flow columns that require alcohol precipitation. Purified plasmid DNA is suitable for automated fluorescent DNA sequencing (typical reads exceed 800 bp), restriction enzyme digestion, ligation, PCR, \u003cem\u003ein vitro\u003c\/em\u003e transcription, transformation, and other applications.\u003c\/p\u003e \u003c\/div\u003e \u003c\/div\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eDownstream Application\u003c\/th\u003e\n\u003ctd\u003eCloning, sequencing, transformation, PCR, restriction digestion, ligation, in vitro transcription etc.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStarting material\u003c\/th\u003e\n\u003ctd\u003e15-50 mL LB culture with OD600 between 2 and 3; or equivalent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePlasmid type\u003c\/th\u003e\n\u003ctd\u003eHigh-copy, low-copy, cosmid DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProcessing mode\u003c\/th\u003e\n\u003ctd\u003eManual (centrifugation or vacuum)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eThroughput\u003c\/th\u003e\n\u003ctd\u003e1 – 24\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDNA binding technology\u003c\/th\u003e\n\u003ctd\u003eSilica Midi Spin Column\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eLysate clearance method\u003c\/th\u003e\n\u003ctd\u003eFiltration using Lysate clearance syringe\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProcessing time\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;40 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eYield\u003c\/th\u003e\n\u003ctd\u003e100-250 µg for high copy-number; 10-50 µg for low copy-number\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecial note\u003c\/th\u003e\n\u003ctd\u003eLysate clearance syringe for rapid sample processing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ensur.omegabio.com\/ensur\/contentAction.aspx?key=Production.2282.S2R4E1A3.20190208.67.4686451\"\u003eProtocol Manual\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ensur.omegabio.com\/ensur\/contentAction.aspx?key=Production.6782.S2R4E1A3.20210528.8.4463327\"\u003eSafety Data Sheet\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Omega Bio-tek","offers":[{"title":"2 Preps","offer_id":45981754753103,"sku":"D6905-00","price":154.0,"currency_code":"ILS","in_stock":true},{"title":"25 Preps","offer_id":45981754785871,"sku":"D6905-03","price":1132.0,"currency_code":"ILS","in_stock":true},{"title":"100 Preps","offer_id":45981754818639,"sku":"D6905-04","price":4097.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/MidiColumn-lysateClearance-D6905-copy.webp?v=1782734785","url":"https:\/\/www.danyel.co.il\/products\/e-z-n-a-fastfilter-plasmid-dna-midi-kit-d6905-00","provider":"danyel biotech","version":"1.0","type":"link"}