{"product_id":"e-z-n-a-endo-free-plasmid-dna-midi-kit-d6915-00","title":"E.Z.N.A.® Endo-Free Plasmid DNA Midi Kit","description":"\u003cdiv class=\"product-summary\"\u003e\u003cul\u003e \u003cli\u003eRapid – Purification of plasmid DNA in \u0026lt; 45 min\u003c\/li\u003e \u003cli\u003eSafe – No phenol\/chloroform extractions\u003c\/li\u003e \u003cli\u003eVersatile – Spin and vacuum formats available\u003c\/li\u003e \u003cli\u003eHigh-quality – Transfection grade, low-endotoxin plasmid DNA (\u0026lt; 1 EU\/µg)\u003c\/li\u003e \u003c\/ul\u003e\u003c\/div\u003e\n\u003cdiv\u003e \u003cdiv\u003e \u003cp\u003eThe E.Z.N.A.® Endo-Free Plasmid DNA Midi Kit provides an efficient method for the large-scale isolation of low-endotoxin plasmid DNA. The system utilizes Lysate Clearance Filter Syringes instead of centrifugation to rapidly clear bacterial lysates post-alkaline lysis. Following lysate clearance, endotoxins are removed, and the protocol follows a simple “bind-wash-elute” procedure to deliver high-quality plasmid DNA. The yields vary according to plasmid copy number, \u003cem\u003eE. coli\u003c\/em\u003e strain, and growth conditions. 15-50 mL of bacterial cultures in LB medium typically produces up to 200 µg of high copy number plasmid DNA. Up to 100 mL of culture may be processed when working with low copy number plasmid. The system uses centrifugation or vacuum technology for plasmid purification and eliminates the time-consuming gravity-flow columns that require alcohol precipitation. Purified plasmid DNA is suitable for sensitive downstream applications such as transfection of mammalian cells, cloning, automated fluorescent DNA sequencing (typical reads exceed 800 bp), next-generation sequencing, restriction enzyme digestion, ligation, PCR, \u003cem\u003ein vitro\u003c\/em\u003e transcription, transformation, etc.\u003c\/p\u003e \u003c\/div\u003e \u003c\/div\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eDownstream Application\u003c\/th\u003e\n\u003ctd\u003eTransfection, gene therapy, cloning, in vitro Transcription, Next-GenerationSequencing, PCR, Transformation, restriction digestion, ligation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStarting material\u003c\/th\u003e\n\u003ctd\u003e15-50 mL LB culture with OD600 between 2 and 3; or equivalent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePlasmid type\u003c\/th\u003e\n\u003ctd\u003eHigh-copy, low-copy, cosmid DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProcessing mode\u003c\/th\u003e\n\u003ctd\u003eManual (centrifugation or vacuum)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eThroughput\u003c\/th\u003e\n\u003ctd\u003e1 – 24\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDNA binding technology\u003c\/th\u003e\n\u003ctd\u003eSilica Midi Spin Column\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eLysate clearance method\u003c\/th\u003e\n\u003ctd\u003eFiltration using Lysate clearance syringe\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProcessing time\u003c\/th\u003e\n\u003ctd\u003e\u0026lt; 45 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eYield\u003c\/th\u003e\n\u003ctd\u003e100-250 µg for high copy-number; 10-50 µg for low copy-number\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecial note\u003c\/th\u003e\n\u003ctd\u003eIncludes endotoxin removal step\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ensur.omegabio.com\/ensur\/contentAction.aspx?key=Production.2287.S2R4E1A3.20190208.67.4695526\"\u003eProtocol Manual\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ensur.omegabio.com\/ensur\/contentAction.aspx?key=Production.6782.S2R4E1A3.20210528.8.4463327\"\u003eSafety Data Sheet\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Omega Bio-tek","offers":[{"title":"2 Preps","offer_id":45981754851407,"sku":"D6915-00","price":228.0,"currency_code":"ILS","in_stock":true},{"title":"25 Preps","offer_id":45981754884175,"sku":"D6915-03","price":1420.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/MidiColumn-endoxin-D6915-copy.webp?v=1782734785","url":"https:\/\/www.danyel.co.il\/products\/e-z-n-a-endo-free-plasmid-dna-midi-kit-d6915-00","provider":"danyel biotech","version":"1.0","type":"link"}