{"product_id":"e-z-n-a-cycle-pure-kit-v-spin-d6492-00","title":"E.Z.N.A.® Cycle Pure Kit (V-spin)","description":"\u003cdiv class=\"product-summary\"\u003e\u003cul\u003e \u003cli\u003eRapid – Purification of PCR products in less than 10 minutes\u003c\/li\u003e \u003cli\u003eSafe – No Phenol\/chloroform extractions\u003c\/li\u003e \u003cli\u003eVersatile – Spin and vacuum formats available\u003c\/li\u003e \u003cli\u003eHigh-quality – DNA is suitable for a variety of downstream applications\u003c\/li\u003e \u003c\/ul\u003e\u003c\/div\u003e\n\u003cdiv\u003e \u003cdiv\u003e \u003cp\u003eThe E.Z.N.A.® Cycle Pure Kit is a convenient system for the fast and reliable purification of PCR products. The E.Z.N.A.® Cycle Pure Kit uses HiBind® technology to recover DNA bands from 100 bp to 10 kb free of oligonucleotides, nucleotides, and polymerase with yields exceeding 80%. The binding conditions of the HiBind® DNA Mini Columns are adjusted by the addition of a specially formulated buffer before adding the sample. Following a rapid wash step, DNA is eluted with deionized water or a low salt buffer. Purified DNA can be directly used for most downstream applications include T-A ligations, PCR sequencing, restriction enzyme digestion, or various labeling reactions.\u003c\/p\u003e \u003c\/div\u003e \u003c\/div\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eDownstream application\u003c\/th\u003e\n\u003ctd\u003eT-A ligations, sequencing, restriction enzyme digestion, and various other labeling reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eElution volume\u003c\/th\u003e\n\u003ctd\u003e30-50 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStarting material\u003c\/th\u003e\n\u003ctd\u003essDNA, dsDNA, PCR products\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStarting amount\u003c\/th\u003e\n\u003ctd\u003e5-50 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDNA recovered\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;80% recovery, 100 bp to 10 kb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProcessing mode\u003c\/th\u003e\n\u003ctd\u003eManual (centrifugation or vacuum)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eThroughput\u003c\/th\u003e\n\u003ctd\u003e1-24\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDNA binding technology\u003c\/th\u003e\n\u003ctd\u003eSilica mini spin column\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProcessing time\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;10 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecial Notes\u003c\/th\u003e\n\u003ctd\u003eFor clean-up of DNA fragments \u0026lt; 200 bp, please follow the protocol modification outlined in the product manual\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ensur.omegabio.com\/ensur\/contentAction.aspx?key=Production.4138.S2R4E1A3.20190923.67.4704238\"\u003eProtocol Manual\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ensur.omegabio.com\/ensur\/contentAction.aspx?key=Production.9409.S2R4E1A3.20230906.294.4924095\"\u003eQuick Guide\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/ensur.omegabio.com\/ensur\/contentAction.aspx?key=Production.6774.S2R4E1A3.20210528.8.4465263\"\u003eSafety Data Sheet\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Omega Bio-tek","offers":[{"title":"5 Preps","offer_id":45981754327119,"sku":"D6492-00","price":80.0,"currency_code":"ILS","in_stock":true},{"title":"50 Preps","offer_id":45981754359887,"sku":"D6492-01","price":335.0,"currency_code":"ILS","in_stock":true},{"title":"200 Preps","offer_id":45981754392655,"sku":"D6492-02","price":844.0,"currency_code":"ILS","in_stock":true},{"title":"600 Preps","offer_id":45981754425423,"sku":"D6492-03","price":3109.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/MiniSpinColumn-withCap-D6492-copy.webp?v=1782734778","url":"https:\/\/www.danyel.co.il\/products\/e-z-n-a-cycle-pure-kit-v-spin-d6492-00","provider":"danyel biotech","version":"1.0","type":"link"}