{"product_id":"e-coli-dna-ligase-2160a","title":"E. coli DNA Ligase","description":"\u003cp\u003eE. coli DNA Ligase catalyzes the formation of phosphodiester bonds between double-stranded DNA fragments with 3'-OH and 5'-phosphate ends, in the presence of NAD+ as the cofactor. Unlike T4 DNA Ligase, E. coli DNA Ligase can only ligate DNA fragments with cohesive ends under standard reaction conditions. E. coli DNA Ligase is supplied in 10 mM potassium phosphate (pH 7.5), 50 mM KCl, 1 mM DTT, 1 mM EDTA and 50% glycerol.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eGene cloning\u003c\/li\u003e\n\u003cli\u003ePhosphodiester bond formation\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eGene cloning\u003cbr\u003eLigation\u003cbr\u003ePhosphodiester bond formation\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/2160A\/2160A.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0328\/SD0328-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/2160A\/2160A_DS.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"1,000 Units","offer_id":45986273493071,"sku":"2160A","price":778.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/2160A-E-coli-DNA-Ligase-product-600.jpg?v=1782807270","url":"https:\/\/www.danyel.co.il\/products\/e-coli-dna-ligase-2160a","provider":"danyel biotech","version":"1.0","type":"link"}