{"product_id":"e-cloni-5-alpha-chemically-competent-cells-comcel-006","title":"E. cloni 5-alpha Chemically Competent Cells","description":"\u003cp\u003eE. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5α™ and directly replace them in cloning protocols. E. cloni chemically competent cells provide the performance researchers need with ease of use—only common laboratory equipment is required. These cells provide solutions for a wide range of applications at economical prices.\u003c\/p\u003e\u003cp\u003eE. cloni 5-alpha Chemically Competent Cells \u0026gt;1 × 10 8 cfu\/µg DNA\u003cbr\u003eUseful for routine cloning, subcloning, and plasmid isolation with or without blue\/white screening.\u003c\/p\u003e\u003cp\u003eTable 1. E. cloni Competent Cells for Cloning\u003c\/p\u003e\u003cp\u003eE. cloni Cell Lines\u003c\/p\u003e\u003cp\u003eTransformation Efficiency\u003cbr\u003e(cfu\/µg pUC DNA)\u003c\/p\u003e\u003cp\u003eCloning Methylated DNA\u003c\/p\u003e\u003cp\u003eBAC, Cosmid Cloning\u003c\/p\u003e\u003cp\u003eBlue\/White Screening\u003c\/p\u003e\u003cp\u003e5-alpha Chemically Competent\u003c\/p\u003e\u003cp\u003e\u0026gt;1 × 10 8 cfu\/µg DNA\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eNO\u003c\/p\u003e\u003cp\u003eYES\u003cbr\u003eNo IPTG induction required\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eDirect replacements for standard cloning strains\u003c\/li\u003e\n\u003cli\u003eOptimised genetics for high yields: endonuclease and recombination minus, for blue\/white screening-capable.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eGenotype\u003c\/p\u003e\u003cp\u003eE. cloni 5-alpha: fhuA2Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17\u003c\/p\u003e\u003cp\u003eEfficiencies\u003c\/p\u003e\u003cp\u003eE. cloni 5-alpha Chemically Competent Cells \u0026gt;1 × 108 cfu\/µg DNA\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eCompetent Cells include Control DNA and Recovery Medium, and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_COMCEL-006_E-cloni-alpha-Chemically-Competent-Cells\"\u003eE cloni alpha Chemically Competent Cells\u003c\/a\u003e\u003c\/li\u003e\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"12 Reactions (DUOs)","offer_id":45986434154575,"sku":"60602-1","price":744.0,"currency_code":"ILS","in_stock":true},{"title":"24 Reactions (DUOs)","offer_id":45986434187343,"sku":"60602-2","price":1273.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/E-cloni-5-alpha-Chemically-Competent-Cells_6246d4d4-8d33-4fe2-8889-23959ca92e0c.jpg?v=1785956158","url":"https:\/\/www.danyel.co.il\/products\/e-cloni-5-alpha-chemically-competent-cells-comcel-006","provider":"danyel biotech","version":"1.0","type":"link"}