{"product_id":"durascribe-t7-transcription-kits-mbtool-007","title":"DuraScribe T7 Transcription Kits","description":"\u003cp\u003eThe DuraScribe® T7 Transcription Kit* is an in vitro transcription kit that produces RNA - called DuraScript® RNA - that is completely resistant to RNase A digestion.\u003c\/p\u003e\u003cp\u003eThe DuraScribe T7 Transcription Kit includes DuraScribe T7 RNA Polymerase, an enhanced formulation of the Y639F mutant 1 of T7 RNA Polymerase that efficiently incorporates 2´-Fluorine-CTP (2´-F-dCTP), 2´-Fluorine-UTP (2´-F-dUTP), ATP and GTP into RNase-resistant RNA transcripts called DuraScript RNA (Figure 1). The DuraScribe T7 RNA Polymerase uses the same T7 promoters as the wild-type T7 RNA Polymerase.\u003c\/p\u003e\u003cp\u003eApplications\u003c\/p\u003e\u003cp\u003eDuraScript RNA has demonstrated advantages for RNA aptamer synthesis, and ribozyme, RNA interference (RNAi) and antisense RNA studies.\u003c\/p\u003e\u003cp\u003eFeatures\u003c\/p\u003e\u003cp\u003eDuraScript RNA is resistant to RNase A and DNase\u003cbr\u003eHigh Yields of DuraScript RNA\u003cbr\u003eT7 in vitro transcription reactions. DuraScribe T7 RNA Polymerase uses the same T7 promoters as the wild type T7 RNA Polymerase.\u003cbr\u003eIdeal for RNA aptamer studies. Numerous citations using the DuraScribe Kit with RNA aptamers\u003cbr\u003eSELEX-compatible. DuraScript RNA can be used in SELEX selection processes.\u003cbr\u003eMake long or short DuraScript RNA. Double-strand oligos, linearized plasmids, and PCR products with a T7 polymerase promoter can be transcribed.\u003c\/p\u003e\u003cp\u003eTable 1. Yield of DuraScript RNA from a DuraScribe Kit reaction. One microgram of a 3-Kb DNA template was linearised at different sites and then transcribed in a DuraScribe T7 Transcription Kit reaction for 4 hours. The yield of DuraScript RNA produced from each template is shown in micrograms (µg) and in picomoles (pmol).\u003c\/p\u003e\u003cp\u003eSize of DuraScript RNA produced\tDuraScript RNA Yield (µg)\tDuraScript RNA Yield (pmol)\u003cbr\u003e2600 nts\t100 µg\t116 pmol\u003cbr\u003e1400 nts\t58 µg\t124 pmol\u003cbr\u003e330 nts\t18 µg\t164 pmol\u003cbr\u003e88 nts\t9 µg\t307 pmol\u003c\/p\u003e\u003cp\u003eFigure 1 (click to enlarge). The DuraScribe T7 RNA Polymerase efficiently incorporates 2´-F-dCTP and 2´-F-dUTP into full-length DuraScript RNA. The presence of the fluorine at the 2´-position of the 2´-F-dC and 2´-F-dU nucleotides prevents digestion by RNase A.\u003c\/p\u003e\u003cp\u003eFigure 2. Yield of RNA from a DuraScribe® T7 Transcription reaction. A standard reaction (4-6 hours) produced 40-60 µg of a 1.4-kb DuraScript® RNA.\u003c\/p\u003e\u003cp\u003eFigure 3. DuraScript® RNA is resistant to RNase A digestion. A 1.4-kb standard RNA transcript and a 1.4-kb DuraScript RNA transcript were each incubated with 1 U of highly purified RNase A for 30 minutes. The standard RNA transcript was completely degraded while the DuraScript RNA transcript remained intact. Lane M, size ladder; lane 1, 1.4-kb standard RNA transcript; lane 2, standard RNA after RNase A treatment; lane 3, 1.4-kb DuraScript RNA; lane 4, DuraScript RNA after RNase A treatment. Denaturing 1% agarose gel 2.\u003c\/p\u003e\u003cp\u003eFigure 4. DuraScript RNA is completely resistant to \"finger\" nucleases. A 1.4-kb standard RNA transcript and a 1.4-kb DuraScript RNA transcript were produced using sterile water or water that had been contaminated by exposure to the hands of a test subject. The standard RNA shows extensive degradation from \"finger\" nucleases in the contaminated water while the DuraScript RNA remains fully intact. M, Size ladder; Lane 1, Standard RNA transcript; Lane 2, Standard RNA after \"finger\" nuclease exposure; Lane 3, DuraScript RNA; Lane 4, DuraScript RNA after \"finger\" nuclease exposure. Denaturing 1% agarose gel 2.\u003c\/p\u003e\u003cp\u003e* Covered by issued and\/or pending patents.\u003c\/p\u003e\u003cp\u003eReferences\u003c\/p\u003e\u003cp\u003eSousa, R. and Padilla, R. (1995) EMBOJ. 14:18 4609-4621.\u003cbr\u003eMolecular Cloning - A Laboratory Manual, Third Edition, 2001. CSHL Press. pp 7.27 - 7.34. J. Sambrook and D. Russell.\u003cbr\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eProduce fully RNase A-resistant RNA that is perfectly suited for RNA aptamer synthesis, as well as antisense RNA and RNA interference (RNAi) experiments.\u003c\/li\u003e\n\u003cli\u003eProtect transcribed RNA from RNAse degradation\u003c\/li\u003e\n\u003cli\u003eProduce high RNA yields\u003c\/li\u003e\n\u003cli\u003eSelect and optimise RNA aptamers using SELEX screening\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eContents: DuraScribe T7 Enzyme Mix, RNase-Free DuraScribe T7 10X Reaction Buffer, ATP, GTP, 2-F-dCTP, 2-F-dUTP, DNase I, DTT, Control Template DNA (linearised), Sterile Deionised Water.\u003c\/p\u003e\n\u003ch2\u003eResources\u003c\/h2\u003e\n\u003cp\u003eAdditional resources\u003c\/p\u003e\u003cp\u003eEXTERNAL LINK\u003c\/p\u003e\u003cp\u003eVector Sequences and Maps\u003c\/p\u003e\n\u003ch2\u003eFAQs\u003c\/h2\u003e\n\u003cp\u003eFAQs\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_MBTOOL-007_Durascribe-t7-transcription-kit\"\u003eDurascribe t7 transcription kit\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_MBTOOL-007_F501-US-DuraScribe-T7-Transcription\"\u003eF501 US DuraScribe T7 Transcription\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"10 Reactions","offer_id":45986440183887,"sku":"DS010910","price":3353.0,"currency_code":"ILS","in_stock":true},{"title":"25 Reactions","offer_id":45986440216655,"sku":"DS010925","price":6030.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/DuraScribe-T7-Transcription-Kits_f6318309-5983-4b97-948b-45a32f24bb6f.jpg?v=1785956218","url":"https:\/\/www.danyel.co.il\/products\/durascribe-t7-transcription-kits-mbtool-007","provider":"danyel biotech","version":"1.0","type":"link"}