{"product_id":"dna-standards-for-library-quantification-638325","title":"DNA Standards for Library Quantification","description":"\u003cp\u003eThe DNA Standards for Library Quantification includes four DNA standards (0.01–10 pM) that are used for qPCR-based library quantification prior to Illumina next-generation sequencing. These DNA standards contain specific concentrations of a DNA fragment (538 bp) and are designed specifically for use with the Library Quantification Kit (Cat No. 638324). When used as templates for qPCR with the Library Quantification Kit, the standards will generate 447-bp DNA fragments that can be used to produce a standard curve for determining the concentration of DNA in an Illumina library. The standards are not suitable for use with other qPCR techniques, as they may lead to quantification errors or poor results.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eHighly sensitive quantification of Illumina sequencing libraries—the qPCR-based assay can measure libraries at low concentrations\u003c\/li\u003e\n\u003cli\u003eAllows specific quantification of only adapter-bound DNA molecules\u003c\/li\u003e\n\u003cli\u003eReduces variability in cluster density to improve sequencing results—use prior to sequencing to ensure optimal loading concentration\u003c\/li\u003e\n\u003cli\u003eComplete kit—includes qPCR primers and reagents, and DNA standards for absolute quantification using a standard curve\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eIllumina NGS library quantification by qPCR\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/638325\/638325.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/ST0289\/ST0289-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/Library%20Quantification%20Kit%20User%20Manual\/Library%20Quantification%20Kit%20User%20Manual_050919.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Components%20List\/638324-050719.pdf\"\u003eLibrary Quantification Kit Components List\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"50 Rxns","offer_id":45986088845391,"sku":"638325","price":1687.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/638325-DNA-Standards-for-Library-Quantification-product-600.jpg?v=1782803731","url":"https:\/\/www.danyel.co.il\/products\/dna-standards-for-library-quantification-638325","provider":"danyel biotech","version":"1.0","type":"link"}