{"product_id":"dna-polymerase-i-large-klenow-fragment-30098-1","title":"DNA Polymerase I Large (Klenow) Fragment","description":"\u003cp\u003eDNA Polymerase I, Large (Klenow) Fragment is a DNA-dependent polymerase that lacks the 5′→3′ exonuclease activities of E. coli DNA Polymerase I from which it is derived. The 3′→5′ remains intact to remove 3’ overhangs, and along with the polymerase activity to fill in 5’ overhangs and create blunt end DNA fragments.  The optimal incubation temperature of the enzyme is 25 °C and uses 10X DNA Polymerase Buffer A. Heat inactivation of the enzyme is 75 °C for 20 minutes.\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eForms blunt ends by removing 3’ overhangs or filling in 5’ overhangs\u003c\/li\u003e\n\u003cli\u003eUsed in second-strand cDNA synthesis\u003c\/li\u003e\n\u003cli\u003eGenerates DNA probes using random primers\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eDNA Polymerase I Large (Klenow) Fragment includes Klenow DNA Polymerase at 5 U\/µL and 10X DNA Polymerase Buffer A\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_30098-1_10X-DNA-Polymerase-Buffer-A\"\u003e10X DNA Polymerase Buffer A\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_30098-1_DNA-Polymerase-I-Large-(Klenow)-Fragment\"\u003eDNA Polymerase I Large (Klenow) Fragment\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_30098-1_DNA-Polymerase-I-Large-(Klenow)-Fragment\"\u003eDNA Polymerase I Large (Klenow) Fragment\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"Default Title","offer_id":45986430320719,"sku":"30098-1","price":1554.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/DNA-Polymerase-I-Large-_2528Klenow_2529-Fragment_54d65d55-83f7-43f2-b901-c3f38638ae12.jpg?v=1785956086","url":"https:\/\/www.danyel.co.il\/products\/dna-polymerase-i-large-klenow-fragment-30098-1","provider":"danyel biotech","version":"1.0","type":"link"}